Cleaved Lamin A (Asp230) Antibody

• 产品编号：CST-2031S      品牌：CST       原厂货号：2031S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

Cleaved Lamin A (Asp230) Antibody 能够检测内源的Asp230位点酶切的lamin A（和C 蛋白）的大片段。此抗体不与全长lamins A发生交叉反应。该多克隆抗体是采用合成的人源lamin A/C蛋白C末端Asp230周围残基相对应的肽段免疫动物而制备的。体由protein A和肽亲和层析技术纯化。

Lamin是核膜的结构组分，对维持正常的细胞功能非常重要，比如细胞周期调控、DNA修复和染色质组装（1-3）。Lamin A/C能够被caspase-6切割，可以作为caspase-6激活的标志。在凋亡过程中，Lamin A/C能够被特异地剪切成大片段(41-50 kDa)和小片段 (28 kDa)（3,4）。Lamin的剪切会引起细胞核的失调和细胞死亡（5，6）。

(This product is sold under license from Promega Corp., U.S. Patent No. 6,350,452.) （此产品经Promega公司许可销售，美国专利号是第6350452号）。

1. Gruenbaum, Y. et al. (2000) J Struct Biol 129, 313-23.
2. Yabuki, M. et al. (1999) Physiol Chem Phys Med NMR 31, 77-84.
3. Goldberg, M. et al. (1999) Crit Rev Eukaryot Gene Expr 9, 285-93.
4. Orth, K. et al. (1996) J Biol Chem 271, 16443-6.
5. Oberhammer, F.A. et al. (1994) J Cell Biol 126, 827-37.
6. Rao, L. et al. (1996) J Cell Biol 135, 1441-55.

原厂资料：

Specificity / Sensitivity

Cleaved Lamin A (Asp230) Antibody detects endogenous levels of the large fragment of lamin A (and lamin C isotype) resulting from cleavage at aspartic acid 230. The antibody does not recognize full length lamin A.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to carboxy-terminal residues surrounding Asp230 in human lamin A. Antibodies are purified by protein A and peptide affinity chromatography.

Background

Lamins are nuclear membrane structural components that are important in maintaining normal cell functions such as cell cycle control, DNA replication and chromatin organization (1-3). Lamin A/C is cleaved by caspase-6 and serves as a marker for caspase-6 activation. During apoptosis, lamin A/C is specifically cleaved into a large (41-50 kDa) and a small (28 kDa) fragment (3,4). The cleavage of lamins results in nuclear disregulation and cell death (5,6).

1. Gruenbaum, Y. et al. (2000) J Struct Biol 129, 313-23.
2. Yabuki, M. et al. (1999) Physiol Chem Phys Med NMR 31, 77-84.
3. Goldberg, M. et al. (1999) Crit Rev Eukaryot Gene Expr 9, 285-93.
4. Orth, K. et al. (1996) J Biol Chem 271, 16443-6.
5. Oberhammer, F.A. et al. (1994) J Cell Biol 126, 827-37.
6. Rao, L. et al. (1996) J Cell Biol 135, 1441-55.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM
NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C.
Do not aliquot the antibody.