Phospho-Lamin A/C (Ser22) Antibody

• 产品编号：CST-2026S      品牌：CST       原厂货号：2026S
• 产品分类：抗体 > 一抗 > 磷酸化抗体
• 应用分类：

描述：

Phospho-Lamin A/C (Ser22)抗体只能用于检测内源性的Ser32磷酸化的lamin A/C蛋白。该多克隆抗体是采用合成的人源lamin A/C蛋白Ser22 周围残基相对应的肽段免疫动物而制备的。Lamin是核膜的结构组分，对维持正常的细胞功能非常重要，比如细胞周期调控、DNA修复和染色质组装（1-3）。Lamin A/C能够被caspase-6切割，可以作为caspase-6激活的标志。在凋亡过程中，Lamin A/C能够被特异地剪切成大片段(41-50 kDa)和小片段 (28 kDa)（3,4）。Lamin的剪切会引起细胞核的失调和细胞死亡（5，6）。用质谱分析技术研究蛋白质组学时发现，多种细胞系中都存在Lamin A/C的Ser22磷酸化。其周围的序列是典型的MAPK/CDK磷酸化基序，这表明它在细胞周期和有丝分裂中发挥作用（7-11)。

1. Gruenbaum, Y. et al. (2000) J Struct Biol 129, 313-23.
2. Yabuki, M. et al. (1999) Physiol Chem Phys Med NMR 31, 77-84.
3. Goldberg, M. et al. (1999) Crit Rev Eukaryot Gene Expr 9, 285-93.
4. Orth, K. et al. (1996) J Biol Chem 271, 16443-6.
5. Oberhammer, F.A. et al. (1994) J Cell Biol 126, 827-37.
6. Rao, L. et al. (1996) J Cell Biol 135, 1441-55.
7. Lowery, D.M. et al. (2007) EMBO J 26, 2262-73.
8. Molina, H. et al. (2007) Proc Natl Acad Sci USA 104, 2199-204.
9. Beausoleil, S.A. et al. (2006) Nat Biotechnol 24, 1285-92.
10. Nousiainen, M. et al. (2006) Proc Natl Acad Sci USA 103, 5391-6.
11. Beausoleil, S.A. et al. (2004) Proc Natl Acad Sci USA 101, 12130-5.

原厂资料：

Specificity / Sensitivity

Phospho-Lamin A/C (Ser22) Antibody detects endogenous levels of lamin A/C only when phosphorylated at Ser22.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser22 of human lamin A/C protein.

Background

Lamins are nuclear membrane structural components that are important in maintaining normal cell functions such as cell cycle control, DNA replication and chromatin organization (1-3). Lamin A/C is cleaved by caspase-6 and serves as a marker for caspase-6 activation. During apoptosis, lamin A/C is specifically cleaved into a large (41-50 kDa) and a small (28 kDa) fragment (3,4). The cleavage of lamins results in nuclear disregulation and cell death (5,6).Phosphorylation of Lamin A/C at Ser22 was identified in vivo in several cell lines by mass spectrometry analysis in proteomic screens. The surrounding sequence is a typical MAPK/CDK phosphorylation motif, which implicates a role in the cell cycle and mitosis (7-11).

1. Gruenbaum, Y. et al. (2000) J Struct Biol 129, 313-23.
2. Yabuki, M. et al. (1999) Physiol Chem Phys Med NMR 31, 77-84.
3. Goldberg, M. et al. (1999) Crit Rev Eukaryot Gene Expr 9, 285-93.
4. Orth, K. et al. (1996) J Biol Chem 271, 16443-6.
5. Oberhammer, F.A. et al. (1994) J Cell Biol 126, 827-37.
6. Rao, L. et al. (1996) J Cell Biol 135, 1441-55.
7. Lowery, D.M. et al. (2007) EMBO J 26, 2262-73.
8. Molina, H. et al. (2007) Proc Natl Acad Sci USA 104, 2199-204.
9. Beausoleil, S.A. et al. (2006) Nat Biotechnol 24, 1285-92.
10. Nousiainen, M. et al. (2006) Proc Natl Acad Sci USA 103, 5391-6.
11. Beausoleil, S.A. et al. (2004) Proc Natl Acad Sci USA 101, 12130-5.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM
NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C.
Do not aliquot the antibody.