Atg12 Antibody (Mouse Specific)

• 产品编号：CST-2011S      品牌：CST       原厂货号：2011S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

Atg12抗体（鼠特异性的）能够检测内源性的游离Atg5和与Atg12结合的Atg5的总蛋白。该单克隆抗体是通过合成肽免疫动物制备的，这些合成肽的序列与鼠源Atg12邻近氨基末端残基相一致。抗体通过多肽亲和色谱法纯化。自噬是自噬溶酶体降解其所包裹的细胞质内容物的一个分解代谢过程(1,2)。通常情况下，自噬在营养匮乏的时候被激活，但它还与许多生理过程相关，包括生长、分化、神经退行性疾病、感染和癌症(3)。自噬的分子机制主要在酵母中发现的，并称之为自噬相关基因。自噬体的形成涉及一个泛素样连接体系，其中Atg12和Atg5共价结合，并定位到自噬体囊泡中(4-6)。上述连接反应由泛素E1样连接酶Atg7和E2样连接酶Atg10介导(7,8)。

1. Reggiori, F. and Klionsky, D.J. (2002) Eukaryot Cell 1, 11-21.
2. Codogno, P. and Meijer, A.J. (2005) Cell Death Differ 12 Suppl 2, 1509-18.
3. Levine, B. and Yuan, J. (2005) J Clin Invest 115, 2679-88.
4. Mizushima, N. et al. (1998) J Biol Chem 273, 33889-92.
5. Mizushima, N. et al. (1998) Nature 395, 395-8.
6. Suzuki, K. et al. (2001) EMBO J 20, 5971-81.
7. Tanida, I. et al. (1999) Mol Biol Cell 10, 1367-79.
8. Shintani, T. et al. (1999) EMBO J 18, 5234-41.

原厂资料：

Specificity / Sensitivity

Atg12 Antibody (Mouse Specific) detects endogenous levels of total free and Atg5 bound Atg12 proteins.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of mouse Atg12. Antibodies are purified by peptide affinity chromatography.

Background

Autophagy is a catabolic process for the autophagosomic-lysosomal degradation of bulk cytoplasmic contents (1,2). Autophagy is generally activated by conditions of nutrient deprivation but has also been associated with a number of physiological processes including development, differentiation, neurodegeneration, infection, and cancer (3). The molecular machinery of autophagy was largely discovered in yeast and referred to as autophagy-related (Atg) genes. Formation of the autophagosome involves a ubiquitin-like conjugation system in which Atg12 is covalently bound to Atg5 and targeted to autophagosome vesicles (4-6). This conjugation reaction is mediated by the ubiquitin E1-like enzyme Atg7 and the E2-like enzyme Atg10 (7,8).

1. Reggiori, F. and Klionsky, D.J. (2002) Eukaryot Cell 1, 11-21.
2. Codogno, P. and Meijer, A.J. (2005) Cell Death Differ 12 Suppl 2, 1509-18.
3. Levine, B. and Yuan, J. (2005) J Clin Invest 115, 2679-88.
4. Mizushima, N. et al. (1998) J Biol Chem 273, 33889-92.
5. Mizushima, N. et al. (1998) Nature 395, 395-8.
6. Suzuki, K. et al. (2001) EMBO J 20, 5971-81.
7. Tanida, I. et al. (1999) Mol Biol Cell 10, 1367-79.
8. Shintani, T. et al. (1999) EMBO J 18, 5234-41.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM
NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C.
Do not aliquot the antibody