BAFF (D7I1U) Rabbit mAb

• 产品编号：CST-19944S      品牌：CST       原厂货号：19944S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

BAFF (D7I1U) Rabbit mAb兔单抗能够检测内源性BAFF总蛋白水平。此单克隆抗体是通过重组人源BAFF蛋白免疫动物制备的。BAFF, TNF超家族的蛋白成员，是一个同源三聚体跨膜蛋白，经过剪切后可以形成可溶性的细胞因子(1)。BAFF 也能进一步的聚合形成60聚体结构(1)。BAFF在中性粒细胞、巨噬细胞、树突状细胞、活化的T细胞和上皮细胞中表达(1,2)。BAFF 在B细胞的发育、存活和激活中发挥了主要的作用(1,3,4)。BAFF结合到3个不同的受体上-BAFF-R、TACI 和 BCMA (1)。这些受体在B细胞及其谱系细胞的发育中具有不同的表达形式(1,2,4)。不像BAFF-R 和BCMA只结合到BAFF的三聚体形式上, TACI只能结合到细胞膜上或者BAFF更高级的结构上(1)。BAFF/ BAFF-R 的相互作用激活典型和非典型的NF-κB信号通路, PI3K/Akt 和 mTOR (2,4)。通过BAFF-R激活非典型的NF-κB信号通路受到TRAF3负调控(5)。研究表明BAFF水平的升高可能加剧许多自身免疫性疾病，这使得它成为治疗的理想靶标(2)。

1. Mackay, F. and Schneider, P. (2009) Nat Rev Immunol 9, 491-502.
2. Moisini, I. and Davidson, A. (2009) Clin Exp Immunol 158, 155-63.
3. Schiemann, B. et al. (2001) Science 293, 2111-4.
4. Khan, W.N. (2009) J Immunol 183, 3561-7.
5. Gardam, S. et al. (2008) Immunity 28, 391-401.

原厂资料：

Specificity / Sensitivity

BAFF (D7I1U) Rabbit mAb recognizes endogenous levels of total BAFF protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with recombinant human BAFF protein.

Background

BAFF, a member of the TNF superfamily of proteins, is a homotrimeric transmembrane protein, which is cleaved to produce a soluble cytokine (1). BAFF may also further oligomerize into 60-mer structures (1). BAFF is expressed by monocytes, neutrophils, macrophages, dendritic cells, activated T cells, and epithelial cells (1,2). BAFF plays a key role in B cell development, survival, and activation (1,3,4). BAFF binds to three distinct receptors, BAFF-R, TACI, and BCMA (1). These receptors are differentially expressed during B cell development and among B cell subsets (1,2,4). While BAFF-R and BCMA bind to the homotrimeric form of BAFF, TACI only binds to membrane bound or higher order BAFF structures (1). The BAFF/ BAFF-R interaction activates both canonical and non-canonical NF-κB pathways, PI3K/Akt, and mTOR (2,4). Activation of the noncanonical NF-κB pathway via BAFF-R is negatively regulated by TRAF3 (5). Research studies have shown that elevated levels of BAFF may exacerbate many autoimmune disorders, making it a potential therapeutic target (2).

1. Mackay, F. and Schneider, P. (2009) Nat Rev Immunol 9, 491-502.
2. Moisini, I. and Davidson, A. (2009) Clin Exp Immunol 158, 155-63.
3. Schiemann, B. et al. (2001) Science 293, 2111-4.
4. Khan, W.N. (2009) J Immunol 183, 3561-7.
5. Gardam, S. et al. (2008) Immunity 28, 391-401.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150
mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02%
sodium azide. Store at –20°C. Do not aliquot the antibody.