OTULIN Antibody

• 产品编号：CST-14127S      品牌：CST       原厂货号：14127S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

Homology

Species predicted to react based on 100% sequence homology: Pig

Specificity / Sensitivity

OTULIN Antibody recognizes endogenous levels of total OTULIN protein. This antibody does not cross-react with OTUB1.

Source / Purification 

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of human OTULIN protein. Antibodies are purified by protein A and peptide affinity chromatography.

原厂资料：

Homology

Species predicted to react based on 100% sequence homology: Pig

Specificity / Sensitivity

OTULIN Antibody recognizes endogenous levels of total OTULIN protein. This antibody does not cross-react with OTUB1.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of human OTULIN protein. Antibodies are purified by protein A and peptide affinity chromatography.

Background

Protein ubiquitination and deubiquitination are reversible processes catalyzed by ubiquitinating enzymes (UBEs) and deubiquitinating enzymes (DUBs) (1,2). Five subfamilies of DUBs have been characterized to date, and include USP, UCH, OTU, MJD, and JAMM deubiquitinating enzymes (1,2). The ovarian tumor (OTU) DUB subfamily comprises a group of approximately 100 putative cysteine proteases that are homologous to the Drosophila ovarian tumor gene product (3). OTU domain-containing deubiquitinase with linear linkage specificity (OTULIN, FAM105B, Gumby) is an OTU subfamily deubiquitinating enzyme that antagonizes the E3 linear ubiquitin chain assembly complex (LUBAC) by promoting disassembly of Met1-linked (linear) ubiquitin chains (4,5). LUBAC and OTULIN regulate NOD2 signaling in an antagonistic manner by controlling the level of Met1-ubiquitinated RIPK2 kinase (6). Binding of the OTULIN PUB-interacting motif to the HOIP subunit of LUBAC is critical for OTULIN inhibition of NF-κΒ signaling; this OTULIN-HOIP interaction is negatively regulated by tyrosine phosphorylation of OTULIN (7,8). The ability of OTULIN to influence LUBAC function and the presence of linear ubiquitin chains may play an important role in regulating angiogenesis, craniofacial, and neural development (5).

1. Nijman, S.M. et al. (2005) Cell 123, 773-86.
2. Nalepa, G. et al. (2006) Nat Rev Drug Discov 5, 596-613.
3. Makarova, K.S. et al. (2000) Trends Biochem Sci 25, 50-2.
4. Keusekotten, K. et al. (2013) Cell 153, 1312-26.
5. Rivkin, E. et al. (2013) Nature 498, 318-24.
6. Fiil, B.K. et al. (2013) Mol Cell 50, 818-30.
7. Elliott, P.R. et al. (2014) Mol Cell 54, 335-48.
8. Schaeffer, V. et al. (2014) Mol Cell 54, 349-61.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM
NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C.
Do not aliquot the antibody