T7-Tag (D9E1X) XP??Rabbit mAb?(HRP Conjugate)

产品编号：CST-14044S 品牌：CST 原厂货号：14044S
产品分类：抗体 > 一抗 > 酶标记一抗 > 辣根过氧化物酶（HRP）
应用分类：

包装：

运保温度:

–20°C

到货周期：

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描述：

Specificity / Sensitivity

T7-Tag (D9E1X) XP^® Rabbit mAb (HRP Conjugate) recognizes transfected levels of recombinant protein containing the T7 epitope tag.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic T7-tag peptide.

Description

This Cell Signaling Technology antibody is conjugated to the carbohydrate groups of horseradish peroxidase (HRP) via its amine groups. The HRP conjugated antibody is expected to exhibit the same species cross-reactivity as the unconjugated T7-Tag (D9E1X) XP^® Rabbit mAb #13246.

原厂资料：

Specificity / Sensitivity

T7-Tag (D9E1X) XP^® Rabbit mAb (HRP Conjugate) recognizes transfected levels of recombinant protein containing the T7 epitope tag.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic T7-tag peptide.

Description

This Cell Signaling Technology antibody is conjugated to the carbohydrate groups of horseradish peroxidase (HRP) via its amine groups. The HRP conjugated antibody is expected to exhibit the same species cross-reactivity as the unconjugated T7-Tag (D9E1X) XP^® Rabbit mAb #13246.

Background

Epitope tags are useful for the labeling and detection of proteins using immunoblotting, immunoprecipitation, and immunostaining techniques. Because of their small size, they are unlikely to affect the tagged protein's biochemical properties.

The T7 tag is an epitope tag composed of an 11-residue peptide encoded from the leader sequence of the T7 bacteriophage gene 10. The T7 tag has been used extensively as a general epitope tag in many expression vectors including the pET system that is based on T7 RNA polymerase expression systems (1).

Studier, F.W. and Moffatt, B.A. (1986) J Mol Biol 189, 113-30.

注意事项：

Storage: Supplied in 136 mM NaCl, 2.6 mM KCl, 12 mM
sodium phosphate (pH 7.4) dibasic, 2 mg/ml BSA, and 50%
glycerol. Store at –20°C. Do not aliquot the antibodies.