Phospho-HS1 (Tyr397) (D12C1) XP??Rabbit mAb?(Pacific Blue? Conjugate)

• 产品编号：CST-13742S      品牌：CST       原厂货号：13742S
• 产品分类：抗体 > 一抗 > 染料标记抗体
• 应用分类：

描述：

Homology

Species predicted to react based on 100% sequence homology: Mouse, Rat

Specificity / Sensitivity

Phospho-HS1 (Tyr397) (D12C1) XP^® Rabbit mAb (Pacific Blue™ Conjugate) recognizes endogenous levels of HS1 protein only when phosphorylated at Tyr397.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Tyr405 of mouse HS1 protein. This site corresponds to Tyr397 of human HS1 protein.

Description

This Cell Signaling Technology antibody is conjugated to Pacific Blue™ fluorescent dye and tested in-house for direct flow cytometry analysis in human cells. The antibody is expected to exhibit the same species cross-reactivity as the unconjugated Phospho-HS1 (Tyr397) (D12C1) XP^® Rabbit mAb #8714.

原厂资料：

Homology

Species predicted to react based on 100% sequence homology: Mouse, Rat

Specificity / Sensitivity

Phospho-HS1 (Tyr397) (D12C1) XP^® Rabbit mAb (Pacific Blue™ Conjugate) recognizes endogenous levels of HS1 protein only when phosphorylated at Tyr397.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Tyr405 of mouse HS1 protein. This site corresponds to Tyr397 of human HS1 protein.

Description

This Cell Signaling Technology antibody is conjugated to Pacific Blue™ fluorescent dye and tested in-house for direct flow cytometry analysis in human cells. The antibody is expected to exhibit the same species cross-reactivity as the unconjugated Phospho-HS1 (Tyr397) (D12C1) XP^® Rabbit mAb #8714.

Background

HS1 (HCLS1, LckBP1, p75) is a protein kinase substrate that is expressed only in tissues and cells of hematopoietic origin (1,2). HS1 contains four cortactin repeats and a single SH3 domain (2). This intracellular protein is phosphorylated following immune receptor activation, which promotes recruitment of HS1 to the immune synapse (3-5). Phosphorylation of HS1 is required to regulate actin dynamics and provide docking sites for many other signaling molecules, such as Vav1 and PLCγ1 (6). HS1 also plays an important role in platelet activation (7).

1. Kitamura, D. et al. (1989) Nucleic Acids Res 17, 9367-79.
2. Kitamura, D. et al. (1995) Biochem Biophys Res Commun 208, 1137-46.
3. Suzuki, H. et al. (1997) J Immunol 159, 5881-8.
4. Hata, D. et al. (1994) Immunol Lett 40, 65-71.
5. Yamanashi, Y. et al. (1993) Proc Natl Acad Sci USA 90, 3631-5.
6. Gomez, T.S. et al. (2006) Immunity 24, 741-52.
7. Kahner, B.N. et al. (2007) Blood 110, 2449-56.

注意事项：

Storage: Supplied in PBS (pH 7.2), less than 0.1% sodium
azide and 2 mg/ml BSA. Store at 4°C. Do not aliquot the
antibody. Protect from light. Do not freeze.