IκBα (L35A5) Mouse mAb (Amino-terminal Antigen) (Pacific Blue? Conjugate)

• 产品编号：CST-13656S      品牌：CST       原厂货号：13656S
• 产品分类：抗体 > 一抗 > 染料标记抗体
• 应用分类：

描述：

Specificity / Sensitivity

IκBα (L35A5) Mouse mAb (Amino-terminal Antigen) (Pacific Blue™ Conjugate) detects endogenous levels of total IκBα protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a GST-IκBα fusion protein corresponding to the amino-terminus of human IκBα protein.

Description

This Cell Signaling Technology antibody is conjugated to Pacific Blue™ fluorescent dye and tested in-house for direct flow cytometry in human cells. The antibody is expected to exhibit the same species cross-reactivity as the unconjugated antibody IκBα (L35A5) Mouse mAb (Amino-terminal Antigen) #4814.

原厂资料：

Specificity / Sensitivity

IκBα (L35A5) Mouse mAb (Amino-terminal Antigen) (Pacific Blue™ Conjugate) detects endogenous levels of total IκBα protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a GST-IκBα fusion protein corresponding to the amino-terminus of human IκBα protein.

Description

This Cell Signaling Technology antibody is conjugated to Pacific Blue™ fluorescent dye and tested in-house for direct flow cytometry in human cells. The antibody is expected to exhibit the same species cross-reactivity as the unconjugated antibody IκBα (L35A5) Mouse mAb (Amino-terminal Antigen) #4814.

Background

The NF-κB/Rel transcription factors are present in the cytosol in an inactive state complexed with the inhibitory IκB proteins (1-3). Activation occurs via phosphorylation of IκBα at Ser32 and Ser36 followed by proteasome-mediated degradation that results in the release and nuclear translocation of active NF-κB (3-7). IκBα phosphorylation and resulting Rel-dependent transcription are activated by a highly diverse group of extracellular signals including inflammatory cytokines, growth factors, and chemokines. Kinases that phosphorylate IκB at these activating sites have been identified (8).

1. Baeuerle, P.A. and Baltimore, D. (1988) Science 242, 540-6.
2. Beg, A.A. and Baldwin, A.S. (1993) Genes Dev 7, 2064-70.
3. Finco, T.S. et al. (1994) Proc Natl Acad Sci USA 91, 11884-8.
4. Brown, K. et al. (1995) Science 267, 1485-8.
5. Brockman, J.A. et al. (1995) Mol Cell Biol 15, 2809-18.
6. Traenckner, E.B. et al. (1995) EMBO J 14, 2876-83.
7. Chen, Z.J. et al. (1996) Cell 84, 853-62.
8. Karin, M. and Ben-Neriah, Y. (2000) Annu Rev Immunol 18, 621-63.

注意事项：

Storage: Supplied in PBS (pH 7.2), less than 0.1% sodium
azide and 2 mg/ml BSA. Store at 4°C. Do not aliquot the
antibody. Protect from light. Do not freeze