Phospho-GKAP (Ser346) Antibody

• 产品编号：CST-13638S      品牌：CST       原厂货号：13638S
• 产品分类：抗体 > 一抗 > 磷酸化抗体
• 应用分类：

描述：

Phospho-GKAP (Ser346) Antibody兔多抗仅能识别Ser346被磷酸化的内源性GKAP蛋白。该多克隆抗体由合成的人GKAP蛋白Ser346邻近的磷酸化肽段免疫动物而获得。抗体通过蛋白A和多肽亲和层析纯化。鸟苷酸激酶相关蛋白(GKAP, DLGAP1 或 SAPAP1)是后突触支架复合物的组分包括PSD-95, SAP90, 和 SHANK蛋白(1-3)。GKAP将突触蛋白SHANK和一个PSD-95复合物结合，该复合物包括NMDA型谷氨酸受体(3,4)。突触的活性引起GKAP被E3泛素连接酶TRIM3泛素化，导致GKAP蛋白被降解而蛋白水平下降(5,6)。GKAP蛋白的降解是被CaMKII依赖的双向机制调节的。突触过度激活导致CaMKII介导的GKAP的Ser346磷酸化，导致GKAP被超泛素化以及支架蛋白从突触被移除。与此相反，低水平的突触活性会使CaMKIIβ磷酸化GKAP，这将触发GKAP从马达蛋白复合物解离，能使GKAP运输到突触的基底并随后掺入到后突触密集区(7)。

1. Satoh, K. et al. (1997) Genes Cells 2, 415-24.
2. Kim, E. et al. (1997) J Cell Biol 136, 669-78.
3. Naisbitt, S. et al. (1999) Neuron 23, 569-82.
4. Romorini, S. et al. (2004) J Neurosci 24, 9391-404.
5. Ehlers, M.D. (2003) Nat Neurosci 6, 231-42.
6. Hung, A.Y. et al. (2010) PLoS One 5, e9842.
7. Shin, S.M. et al. (2012) Nat Neurosci 15, 1655-66.

原厂资料：

omology

Species predicted to react based on 100% sequence homology: Human

Specificity / Sensitivity

Phospho-GKAP (Ser346) Antibody recognizes endogenous levels of GKAP protein only when phosphorylated at Ser346.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser346 of human GKAP protein. Antibodies are purified by protein A and peptide affinity chromatography.

Background

Guanylate kinase-associated protein (GKAP, DLGAP1 or SAPAP1) is part of the postsynaptic scaffolding complex that includes the PSD-95, SAP90, and SHANK proteins (1-3). GKAP links the synaptic protein SHANK to a PSD-95 complex that includes NMDA glutamate receptors (3,4). Synaptic activity induces ubiquitination of GKAP protein by the E3 ubiquitin ligase TRIM3, which results in decreased GKAP protein levels through degradation (5,6). GKAP protein turnover is regulated by a CaMKII-dependent, bidirectional mechanism. Synaptic over-excitation leads to CaMKIIα-mediated GKAP phosphorylation at Ser346, which induces polyubiquitination of GKAP and removal of the scaffold protein from synapses. In contrast, during low-level synaptic activity CaMKIIβ phosphorylates GKAP, which triggers dissociation of GKAP from the motor protein complex responsible for GKAP transport to the base of the synapse and its subsequent incorporation into the postsynaptic density (7).

1. Satoh, K. et al. (1997) Genes Cells 2, 415-24.
2. Kim, E. et al. (1997) J Cell Biol 136, 669-78.
3. Naisbitt, S. et al. (1999) Neuron 23, 569-82.
4. Romorini, S. et al. (2004) J Neurosci 24, 9391-404.
5. Ehlers, M.D. (2003) Nat Neurosci 6, 231-42.
6. Hung, A.Y. et al. (2010) PLoS One 5, e9842.
7. Shin, S.M. et al. (2012) Nat Neurosci 15, 1655-66.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM
NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C.
Do not aliquot the antibody.