DNA Polymerase γ (D1Y6R) Rabbit mAb

• 产品编号：CST-13609S      品牌：CST       原厂货号：13609S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

DNA Polymerase γ (D1Y6R) Rabbit mAb可以识别内源性的DNA Polymerase γ 总蛋白。单克隆抗体由合成肽段免疫动物产生，该肽段与人DNA polymerase γ蛋白的Pro560邻近氨基酸序列一致。DNA聚合酶γ (POLG, pol gamma)是一个细胞核编码的蛋白，负责真核细胞中线粒体基因组的复制。140kDa的聚合酶γ催化亚基和55 kDa的附件蛋白(POLG2, pol γB)形成二聚体，获得持续合成能力（1）。除了聚合酶功能，聚合酶γ包含用于校对的3'-5'外切酶活性和5'-脱氧核糖磷酸合酶活性的DNA碱基切除修复（BER）的功能。DNA修复时催化亚基识别损伤DNA的比例是由pol γB附件亚基来增强的（2）。对应的POLG基因发生突变后会发生若干遗传性神经疾病包括进行性外眼肌麻痹，myocerebrohepatopathy谱系障碍和阿尔珀斯-Huttenlocher综合征(3,4)。研究指出POLG基因发生突变可能会促进乳腺肿瘤的形成（5）。

1. Kaguni, L.S. (2004) Annu Rev Biochem 73, 293-320.
2. Pinz, K.G. and Bogenhagen, D.F. (2006) DNA Repair (Amst) 5, 121-8.
3. Stumpf, J.D. et al. (2013) Cold Spring Harb Perspect Biol 5, a011395.
4. Finsterer, J. (2011) J Neurol Sci 304, 9-16.
5. Singh, K.K. et al. (2009) J Hum Genet 54, 516-24.

原厂资料：

Specificity / Sensitivity

DNA Polymerase γ (D1Y6R) Rabbit mAb recognizes endogenous levels of total DNA polymerase γ protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro560 of human DNA polymerase γ protein.

Background

DNA polymerase γ (POLG, pol gamma) is a nuclear encoded protein that is responsible for mitochondrial genome replication in eukaryotic cells. The 140 kDa polymerase γ catalytic subunit forms a holoenzyme complex with a 55 kDa accessory protein (POLG2, pol γB) dimer, which confers processivity (1). In addition to polymerase activity, polymerase γ contains 3'-5' exonuclease activity for proofreading and 5′-deoxyribonucleic phosphate lyase activity that functions in DNA base excision repair (BER). The rate at which the catalytic subunit recognizes damaged DNA during DNA repair is enhanced by the pol γB accessory subunit (2). Mutations in the corresponding POLG gene are associated with several inherited neuropathies including progressive external ophthalmoplegia, myocerebrohepatopathy spectrum disorders and Alpers-Huttenlocher syndrome (3,4). Research studies indicate that mutations in the corresponding POLG gene may promote breast tumorigenesis (5).

1. Kaguni, L.S. (2004) Annu Rev Biochem 73, 293-320.
2. Pinz, K.G. and Bogenhagen, D.F. (2006) DNA Repair (Amst) 5, 121-8.
3. Stumpf, J.D. et al. (2013) Cold Spring Harb Perspect Biol 5, a011395.
4. Finsterer, J. (2011) J Neurol Sci 304, 9-16.
5. Singh, K.K. et al. (2009) J Hum Genet 54, 516-24.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150
mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02%
sodium azide. Store at –20°C. Do not aliquot the antibody