Myosin VI (D5Y5A) Rabbit mAb

• 产品编号：CST-13592S      品牌：CST       原厂货号：13592S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

</p>Myosin VI (D5Y5A) Rabbit mAb可以识别内源性总的myosin VI蛋白。</p>单克隆抗体由合成肽段免疫动物产生，该肽段与人myosin VI蛋白Arg1131附近氨基酸一致。</p>马达蛋白myosin家族真核细胞中推动ATP-依赖的actin-based运动，myosin家族含有一个保守的氨基末端马达结构域（1,2）。Myosin VI是一个非传统的minus-edn-directedmyosin涉及了囊泡和细胞器的转运，内吞和细胞器生成（3-6）。myosin VI的运动和沿着肌动蛋白丝的cargo在myosin家族成员中是一种独特的机制；它的尾部结构域使其能够采取大于预测的步骤沿着肌动蛋白丝运动（1,7）。Myosin VI也被证明可以调控蛋白的极化分布到特异的亚细胞位置，包括促进EGFR到达迁移细胞的前缘，以及运送神经元中专门的轴突蛋白（9）。Myosin VI也被证明接到了DNA损伤过程中抑癌因子p53的活性（10,11）。

1. Sweeney, H.L. and Houdusse, A. (2010) Cell 141, 573-82.
2. Nambiar, R. et al. (2010) Cell Mol Life Sci 67, 1239-54.
3. Bond, L.M. et al. (2011) Mol Biol Cell 22, 54-65.
4. Aschenbrenner, L. et al. (2004) Mol Biol Cell 15, 2253-63.
5. Loubéry, S. et al. (2012) Traffic 13, 665-80.
6. Ameen, N. and Apodaca, G. (2007) Traffic 8, 998-1006.
7. Spudich, J.A. and Sivaramakrishnan, S. (2010) Nat Rev Mol Cell Biol 11, 128-37.
8. Chibalina, M.V. et al. (2010) Traffic 11, 1290-303.
9. Lewis, T.L. et al. (2011) PLoS Biol 9, e1001021.
10. Jung, E.J. et al. (2006) Mol Cell Biol 26, 2175-86.
11. Cho, S.J. and Chen, X. (2010) J Biol Chem 285, 27159-66.

原厂资料：

Specificity / Sensitivity

<p>Myosin VI (D5Y5A) Rabbit mAb recognizes endogenous levels of total myosin VI protein.

Source / Purification

<p>Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Arg1131 of human myosin VI protein.

Background

<p>The myosin family of motor proteins drive ATP-dependent actin-based motility in eukaryotic cells and contain a conserved amino-terminal motor domain (reviewed in 1,2).</p><p>Myosin VI is an unconventional minus-end-directed myosin involved in the transport of vesicles and organelles within the cell, endocytosis, and organelle biogenesis (3-6). The movement of myosin VI and its cargo along actin filaments is unique among myosin family members in its mechanism; its tail domain structure allows it to take larger than predicted steps along the actin filament (reviewed in 1,7).</p><p>Myosin VI has been shown to regulate the polarized delivery of proteins to specialized subcellular locations, including the delivery of EGFR to the leading edge of migrating cells (8), as well as the delivery of specialized axonal proteins in neurons (9). Myosin VI has also been shown to mediate activity of the tumor suppressor p53 during DNA damage (10,11).

1. Sweeney, H.L. and Houdusse, A. (2010) Cell 141, 573-82.
2. Nambiar, R. et al. (2010) Cell Mol Life Sci 67, 1239-54.
3. Bond, L.M. et al. (2011) Mol Biol Cell 22, 54-65.
4. Aschenbrenner, L. et al. (2004) Mol Biol Cell 15, 2253-63.
5. Loubéry, S. et al. (2012) Traffic 13, 665-80.
6. Ameen, N. and Apodaca, G. (2007) Traffic 8, 998-1006.
7. Spudich, J.A. and Sivaramakrishnan, S. (2010) Nat Rev Mol Cell Biol 11, 128-37.
8. Chibalina, M.V. et al. (2010) Traffic 11, 1290-303.
9. Lewis, T.L. et al. (2011) PLoS Biol 9, e1001021.
10. Jung, E.J. et al. (2006) Mol Cell Biol 26, 2175-86.
11. Cho, S.J. and Chen, X. (2010) J Biol Chem 285, 27159-66.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150
mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02%
sodium azide. Store at –20°C. Do not aliquot the antibody