Mios (D12C6) Rabbit mAb

• 产品编号：CST-13557S      品牌：CST       原厂货号：13557S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

Mios (D12C6) Rabbit mAb兔单抗可以识别内源性总的Mios蛋白。单克隆抗体由合成肽段免疫动物产生，该肽段与人Mios的Leu730邻近残基序列一致。mTORC1激酶复合物在细胞生长调节过程中发挥了关键作用（1,2）。mTORC1活性能被能能量水平，生长因子和氨基酸水平调节（3,4）。4个相关的GTPases (RagA, RagB, RagC, 和 RagD)可以和mTORC1中的raptor结合，这是氨基酸信号引起的mTORC1活化的充分必要条件（1,2）。GAP 针对Rags (GATOR) 复合物的活性通过和Rag GTPases相互作用而实现，这个复合物由一组蛋白亚复合物构成（5）。GATOR1亚复合物包括蛋白DEPDC5, Nprl2 和Nprl3，它是一个RagA和RagB GTPase激活蛋白（GAP）能负调控mTORC1信号。相反，GATOR2亚复合物（包括Mios, WDR24, WDR59, Seh1L 和 Sec13）则是mTORC1的正向调控因子（5）。mTORC1激酶复合物在细胞生长调节过程中发挥了关键作用（1,2）。mTORC1活性能被能能量水平，生长因子和氨基酸水平调节（3,4）。4个相关的GTPases (RagA, RagB, RagC, 和 RagD)可以和mTORC1中的raptor结合，这是氨基酸信号引起的mTORC1活化的充分必要条件（1,2）。GAP 针对Rags (GATOR) 复合物的活性通过和Rag GTPases相互作用而实现，这个复合物由一组蛋白亚复合物构成（5）。GATOR1亚复合物包括蛋白DEPDC5, Nprl2 和Nprl3，它是一个RagA和RagB GTPase激活蛋白（GAP）能负调控mTORC1信号。相反，GATOR2亚复合物（包括Mios, WDR24, WDR59, Seh1L 和 Sec13）则是mTORC1的正向调控因子（5）。

1. Sancak, Y. et al. (2008) Science 320, 1496-501.
2. Kim, E. et al. (2008) Nat Cell Biol 10, 935-45.
3. Hay, N. and Sonenberg, N. (2004) Genes Dev 18, 1926-45.
4. Wullschleger, S. et al. (2006) Cell 124, 471-84.
5. Bar-Peled, L. et al. (2013) Science 340, 1100-6.
6. Iida, T. and Lilly, M.A. (2004) Development 131, 1029-39.

原厂资料：

Specificity / Sensitivity

Mios (D12C6) Rabbit mAb recognizes endogenous levels of total Mios protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Leu730 of human Mios protein.

Background

The mTORC1 kinase complex plays a critical role in cell growth regulation (1,2). mTORC1 activity is modulated by energy levels, growth factors, and amino acids (3,4). Four related GTPases (RagA, RagB, RagC, and RagD) interact with raptor in mTORC1, which is necessary and sufficient for mTORC1 activation in response to amino acid signals (1,2). The GAP activity towards Rags (GATOR) complex interacts with Rag GTPases and is made up of a pair of protein subcomplexes (5). The GATOR1 subcomplex includes the proteins DEPDC5, Nprl2 and Nprl3, and is a RagA and RagB GTPase-activating protein (GAP) that negatively regulates mTORC1 signaling. Conversely, the GATOR2 subcomplex (including Mios, WDR24, WDR59, Seh1L and Sec13 proteins) is a positive regulator of mTORC1 signaling (5).The mammalian Mios protein contains six amino-terminal WD repeats and a carboxy terminal zinc finger-like domain. The homologous Drosophila missing oocyte (Mio) protein is required for meiotic progression and oocyte cell fate maintenance during development (6).

1. Sancak, Y. et al. (2008) Science 320, 1496-501.
2. Kim, E. et al. (2008) Nat Cell Biol 10, 935-45.
3. Hay, N. and Sonenberg, N. (2004) Genes Dev 18, 1926-45.
4. Wullschleger, S. et al. (2006) Cell 124, 471-84.
5. Bar-Peled, L. et al. (2013) Science 340, 1100-6.
6. Iida, T. and Lilly, M.A. (2004) Development 131, 1029-39.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150
mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02%
sodium azide. Store at –20°C. Do not aliquot the antibody