Rad23B (D4W7F) Rabbit mAb

• 产品编号：CST-13525S      品牌：CST       原厂货号：13525S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

Rad23B (D4W7F) Rabbit mAb兔单抗识别内源性的Rad23B总蛋白。此抗体不与Rad23A蛋白发生交叉反应。此单克隆抗体经合成人Rad23B蛋白Ala140邻近的氨基酸残基序列一致的肽段，免疫动物产生。酵母中的核苷酸切除修复(NER)辐射敏感蛋白23(rad23)及其人同源体Rad23A(hHR23A)和Rad23B(hHR23B)是细胞识别DNA损伤的重要的组件，并且可作为受体，转运目标泛素化底物到蛋白酶体降解(1)。紫外线切除修复蛋白质Rad23B是一种具有多种结构域的支架蛋白，它在依赖泛素化的蛋白降解过程中扮演重要角色。Rad23B氨基末端含有一个ubiquitin-like(UbL)领域，介导其与蛋白酶体19S调控复合体的S5a / PSMD4亚基相互作用(2，3)。此外，Rad23B包含一个中心泛素化相关结构域(UBA1)和一个羰基末端UBA2域, 分别结合单泛素基团或多泛素基团(4)。研究表明，Rad23B特异性结合K48位点泛素化协助蛋白酶体招募目标蛋白(5)。在成对的UBA结构域间,Rad23B包含一个XPC结合结构域,协助其结合XPC和DNA损伤(6),以及肽的绑定:N聚糖酶对蛋白酶体招募泛素化的ERAD底物至关重要(7)。研究表明, 小鼠Rad23B位点缺失损害胚胎发育,表明Rad23B对哺乳动物的发育至关重要的(8)。

1. Verma, R. et al. (2004) Cell 118, 99-110.
2. Ryu, K.S. et al. (2003) J Biol Chem 278, 36621-7.
3. Walters, K.J. et al. (2003) Proc Natl Acad Sci U S A 100, 12694-9.
4. Raasi, S. et al. (2005) Nat Struct Mol Biol 12, 708-14.
5. Nathan, J.A. et al. (2013) EMBO J 32, 552-65.
6. Masutani, C. et al. (1994) EMBO J 13, 1831-43.
7. Lee, J.H. et al. (2005) Proc Natl Acad Sci U S A 102, 9144-9.
8. Ng, J.M. et al. (2002) Mol Cell Biol 22, 1233-45.

原厂资料：

Specificity / Sensitivity

Rad23B (D4W7F) Rabbit mAb recognizes endogenous levels of total Rad23B protein. This antibody does not cross-react with Rad23A protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ala140 of human Rad23B protein.

Background

The yeast nucleotide excision repair (NER) radiation sensitive protein 23 (rad23) and its human homologs Rad23A (hHR23A) and Rad23B (hHR23B) are critical components of the cellular machinery that recognize DNA lesions and serve as receptors that target ubiquitinated substrates to the proteasome for degradation (1).The UV excision repair protein Rad23B is a multi-domain scaffold protein that plays an important role in ubiquitin-dependent proteasomal degradation. Rad23B contains an amino-terminal ubiquitin-like (UbL) domain that facilitates interaction with the S5a/PSMD4 subunit of the proteasome 19S regulatory complex (2,3). In addition, Rad23B contains a central ubiquitin-associated domain (UBA1) and a carboxy-terminal UBA2 domain, which bind mono- and polyubiquitin with distinct specificities (4). Research studies demonstrate that Rad23B binds specifically to K48-ubiquitinated proteins to facilitate recruitment of target proteins to the proteasome (5). Between the paired UBA domains, Rad23B contains an XPC-binding domain that facilitates binding to XPC and recruitment to DNA lesions (6), as well as the binding of peptide:N-glycanase that is critical for recruitment of ubiquitinated ERAD substrates to the proteasome (7). Research studies have shown that targeted deletion of the murine Rad23b locus impairs embryonic development, suggesting that Rad23B is essential for mammalian development (8).

1. Verma, R. et al. (2004) Cell 118, 99-110.
2. Ryu, K.S. et al. (2003) J Biol Chem 278, 36621-7.
3. Walters, K.J. et al. (2003) Proc Natl Acad Sci U S A 100, 12694-9.
4. Raasi, S. et al. (2005) Nat Struct Mol Biol 12, 708-14.
5. Nathan, J.A. et al. (2013) EMBO J 32, 552-65.
6. Masutani, C. et al. (1994) EMBO J 13, 1831-43.
7. Lee, J.H. et al. (2005) Proc Natl Acad Sci U S A 102, 9144-9.
8. Ng, J.M. et al. (2002) Mol Cell Biol 22, 1233-45.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150
mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02%
sodium azide. Store at –20°C. Do not aliquot the antibody