Spartin Antibody

• 产品编号：CST-13520S      品牌：CST       原厂货号：13520S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

Spartin抗体可以识别内源性Spartin总蛋白。多抗由合成肽段免疫动物产生，该肽段与人spartin蛋白氨基端肽段序列一致。抗体由蛋白A和肽段亲和层析技术纯化得到。Spastic paraplegia 20（spartin）由人类SPG20基因编码，在某些个体中会发生变化产生一种常染色体隐性遗传形式的spastic paraplegia被称为泰勒综合征（1,2）。对泰勒综合症的研究已经清楚的描述了spartin的细胞定位或功能，更多的工作表明spartin在内体运输，微管动力学和脂质环境稳态中发挥功能（3-5）。这种多功能的蛋白广泛表达在神经系统和非神经性组织中，并表现了不同的细胞定位（6）。在多个结直肠癌病例中，SPG20基因启动子高度甲基化，导致了spartin蛋白表达下降以及胞质分裂停滞。这提示spartin的表达和甲基化状态可能是多种结直肠癌的重要生物标记（7）。

1. Patel, H. et al. (2002) Nat Genet 31, 347-8.
2. Bakowska, J.C. et al. (2008) Arch Neurol 65, 520-4.
3. Edwards, T.L. et al. (2009) Biochem J 423, 31-9.
4. Lu, J. et al. (2006) J Neurochem 98, 1908-19.
5. Eastman, S.W. et al. (2009) J Cell Biol 184, 881-94.
6. Milewska, M. et al. (2009) J Neurochem 111, 1022-30.
7. Lind, G.E. et al. (2011) Oncogene 30, 3967-78.

原厂资料：

Specificity / Sensitivity

Spartin Antibody recognizes endogenous levels of total spartin protein.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of human spartin protein. Antibodies are purified by protein A and peptide affinity chromatography. 

Background

Spastic paraplegia 20 (spartin) is encoded by the SPG20 gene in humans, which is altered in some individuals with an autosomal recessive form of hereditary spastic paraplegia known as Troyer syndrome (1,2). While Troyer syndrome research studies have yet to clearly describe the subcellular localization or function of spartin, additional work implicates spartin in endosomal trafficking, microtubule dynamics, and lipid homoeostasis (3-5). This multifunctional protein is ubiquitously expressed within the nervous system and in non-neuronal tissues, and displays a diverse pattern of cellular localization (6). The SPG20 gene promoter is hypermethylated in many cases of colorectal cancer, which results in decreased spartin expression and cytokinesis arrest. This suggests that spartin expression and methylation state could be a promising biomarker for colorectal tumors (7).

1. Patel, H. et al. (2002) Nat Genet 31, 347-8.
2. Bakowska, J.C. et al. (2008) Arch Neurol 65, 520-4.
3. Edwards, T.L. et al. (2009) Biochem J 423, 31-9.
4. Lu, J. et al. (2006) J Neurochem 98, 1908-19.
5. Eastman, S.W. et al. (2009) J Cell Biol 184, 881-94.
6. Milewska, M. et al. (2009) J Neurochem 111, 1022-30.
7. Lind, G.E. et al. (2011) Oncogene 30, 3967-78.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM
NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C.
Do not aliquot the antibody