LXR-β (D6M9D) Rabbit mAb

• 产品编号：CST-13519S      品牌：CST       原厂货号：13519S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

LXR-β (D6M9D) Rabbit mAb可以识别内源性LXR-β总蛋白水平。单抗由合成肽段免疫动物获得，肽段和人LXR-β蛋白的氨基端序列一致。Liver X receptors LXR-α和LXR-β是细胞核激素受体超家族成员，通过控制胆固醇的转运和代谢调控靶基因的表达(1)。当胆固醇(氧化型胆固醇)的衍生物结合后，激活的LXR受体作为甾醇传感器调节参与胆固醇动态平衡的基因的转录(1,2)。LXR-α在大鼠的肝脏，肾脏，小肠，脂肪组织和脾脏中高表达；LXR-β更广泛的表达在各种大鼠组织中(1,3)。研究指出葡萄糖能够结合并上调LXR-α 和LXR-β的转录活性(4)。LXR-α 和LXR-β是潜在的葡萄糖感受器，调控肝脏中葡萄糖代谢和脂肪酸合成(4)。还有研究显示雌性小鼠LXR-β缺陷会形成胆囊癌(5)。此外，LXR-β在帕金森病模型中发挥了保护多巴胺能神经元的功能(6)。

1. Repa, J.J. et al. (2000) Genes Dev 14, 2819-30.
2. Willy, P.J. et al. (1995) Genes Dev 9, 1033-45.
3. Teboul, M. et al. (1995) Proc Natl Acad Sci U S A 92, 2096-100.
4. Mitro, N. et al. (2007) Nature 445, 219-23.
5. Gabbi, C. et al. (2010) Proc Natl Acad Sci U S A 107, 14763-8.
6. Dai, Y.B. et al. (2012) Proc Natl Acad Sci U S A 109, 13112-7.

原厂资料：

Specificity / Sensitivity

LXR-β (D6M9D) Rabbit mAb recognizes endogenous levels of total LXR-β protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of human LXR-β protein.

Background

Liver X receptors LXR-α and LXR-β are nuclear hormone receptor superfamily members responsible for regulating expression of target genes that control cholesterol transport and metabolism (1). When bound by the oxidized derivatives of cholesterol (oxysterols), activated LXR receptors function as sterol sensors to regulate transcription of the genes involved in the cholesterol homeostasis (1,2). The LXR-α protein is expressed at high levels in rat liver, kidney, intestine, adipose, and spleen; LXR-β is more ubiquitously expressed within rat tissues (1,3). Research studies indicate that glucose binds and up-regulates the transcriptional activity of LXR-α and LXR-β (4). LXR-α and LXR-β are putative glucose sensors that integrate glucose metabolism and fatty acid biosynthesis in the liver (4). Additional studies show that female mice deficient in LXR-β develop gallbladder cancer (5). In addition, LXR-β plays a role in protecting dopaminergic neurons in a Parkinson disease model (6).

1. Repa, J.J. et al. (2000) Genes Dev 14, 2819-30.
2. Willy, P.J. et al. (1995) Genes Dev 9, 1033-45.
3. Teboul, M. et al. (1995) Proc Natl Acad Sci U S A 92, 2096-100.
4. Mitro, N. et al. (2007) Nature 445, 219-23.
5. Gabbi, C. et al. (2010) Proc Natl Acad Sci U S A 107, 14763-8.
6. Dai, Y.B. et al. (2012) Proc Natl Acad Sci U S A 109, 13112-7.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150
mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02%
sodium azide. Store at –20°C. Do not aliquot the antibody.