Atg101 (E1Z4W) Rabbit mAb

• 产品编号：CST-13492S      品牌：CST       原厂货号：13492S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

Atg101 (E1Z4W) Rabbit mAb 兔单抗能够识别内源性Atg101总蛋白。该单克隆抗体通过合成肽免疫动物制备，这些合成肽序列与人源Atg101蛋白 Atg177 位点周围的残基相一致。Atg101作为Atg13结合蛋白被发现，同时作为ULK1 丝氨酸-苏氨酸激酶复合体的一个部分，参与自噬(1-3)。自噬是自噬溶酶体对其包裹的细胞质内含物进行降解的一种分解代谢过程(4,5)。自噬过程一般是在营养匮乏的条件下被激活，但是也与一些生理过程有关，如发育、分化、神经变性、感染和癌症(6)。在酵母中已阐明自噬的分子机制，是由许多自噬相关基因(Atg)调控。ULK1包含 Atg13和FIP200的复合体，是饥饿诱导的自噬所必需的(7-9)。Atg101和Atg13的相互作用，对Atg13和ULK1的稳定性和底物磷酸化是重要的(1,2)。

1. Mercer, C.A. et al. (2009) Autophagy 5, 649-62.
2. Hosokawa, N. et al. (2009) Autophagy 5, 973-9.
3. Chan, E.Y. et al. (2007) J Biol Chem 282, 25464-74.
4. Reggiori, F. and Klionsky, D.J. (2002) Eukaryot Cell 1, 11-21.
5. Codogno, P. and Meijer, A.J. (2005) Cell Death Differ 12 Suppl 2, 1509-18.
6. Levine, B. and Yuan, J. (2005) J Clin Invest 115, 2679-88.
7. Hosokawa, N. et al. (2009) Mol Biol Cell 20, 1981-91.
8. Jung, C.H. et al. (2009) Mol Biol Cell 20, 1992-2003.
9. Ganley, I.G. et al. (2009) J Biol Chem 284, 12297-305.

原厂资料：

Specificity / Sensitivity

Atg101 (E1Z4W) Rabbit mAb recognizes endogenous levels of total Atg101 protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Val177 of human Atg101 protein.

Background

Atg101 was discovered as a binding protein for Atg13, a component of the ULK1 serine-threonine kinase complex required for autophagy (1-3). Autophagy is a catabolic process for the autophagosomic-lysosomal degradation of bulk cytoplasmic contents (4,5). It is generally activated by conditions of nutrient deprivation, but is also associated with a number of physiological processes including development, differentiation, neurodegeneration, infection, and cancer (6). The molecular machinery of autophagy was largely discovered in yeast and is directed by a number of autophagy-related (Atg) genes. The ULK1 complex includes both Atg13 and FIP200 and is required for starvation-induced autophagy (7-9). Interaction between Atg101 and Atg13 can be important for the stability and basal phosphorylation of Atg13 and ULK1 (1,2).

1. Mercer, C.A. et al. (2009) Autophagy 5, 649-62.
2. Hosokawa, N. et al. (2009) Autophagy 5, 973-9.
3. Chan, E.Y. et al. (2007) J Biol Chem 282, 25464-74.
4. Reggiori, F. and Klionsky, D.J. (2002) Eukaryot Cell 1, 11-21.
5. Codogno, P. and Meijer, A.J. (2005) Cell Death Differ 12 Suppl 2, 1509-18.
6. Levine, B. and Yuan, J. (2005) J Clin Invest 115, 2679-88.
7. Hosokawa, N. et al. (2009) Mol Biol Cell 20, 1981-91.
8. Jung, C.H. et al. (2009) Mol Biol Cell 20, 1992-2003.
9. Ganley, I.G. et al. (2009) J Biol Chem 284, 12297-305.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150
mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02%
sodium azide. Store at –20°C. Do not aliquot the antibody.