Ubiquityl-PCNA (Lys164) (D5C7P) Rabbit mAb

• 产品编号：CST-13439S      品牌：CST       原厂货号：13439S
• 产品分类：抗体 > 一抗 > 其它类型一抗
• 应用分类：

描述：

Ubiquityl-PCNA (Lys164) (D5C7P) Rabbit mAb可以识别Lys164被泛素化的内源性PCNA蛋白。单克隆抗体由合成肽段免疫动物产生，该肽段与人PCNA的Lys164邻近残基序列对应。增殖细胞核抗原（PCNA）是DNA滑动钳蛋白家族的一员，协助DNA复制（1）。晶体结构数据提示PCNA形成的同源三聚体环可以环绕包围DNA双螺旋（2）。很多涉及到DNA复制，修复和细胞周期调控的蛋白都是与PCNA结合而不是直接地和DNA结合，因此可以促进DNA的快速处理（reviewed in 3）。PCNA蛋白表达是一个广泛认可的增殖信号，PCNA（PC10）mouse mAb已经被上百种研究用于衡量PCNA的表达量。停滞的复制叉发生DNA损伤后，PCNA的Lys164会被单泛素化。这个修饰对于跨损伤修复（TLS）DNA修复途径是很重要的(reviewed in 4,5).

1. Kelman, Z. and O'Donnell, M. (1995) Nucleic Acids Res 23, 3613-20.
2. Krishna, T.S. et al. (1994) Cell 79, 1233-43.
3. Maga, G. and Hubscher, U. (2003) J Cell Sci 116, 3051-60.
4. Lee, K.Y. and Myung, K. (2008) Mol Cells 26, 5-11.
5. Chen, J. et al. (2011) Cell Biochem Biophys 60, 47-60.

原厂资料：

Specificity / Sensitivity

Ubiquityl-PCNA (Lys164) (D5C7P) Rabbit mAb recognizes endogenous levels of PCNA protein only when ubiquitinated at Lys164.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Lys164 of human PCNA protein.

Background

Proliferating cell nuclear antigen (PCNA) is a member of the DNA sliding clamp family of proteins that assist in DNA replication (1). Crystal structure data suggests that a PCNA homotrimer ring can encircle and slide along the DNA double helix (2). Multiple proteins involved in DNA replication, DNA repair, and cell cycle control bind to PCNA rather than directly associating with DNA, thus facilitating fast processing of DNA (reviewed in 3). PCNA protein expression is a well-accepted marker of proliferation and PCNA (PC10) mouse mAb has been used to assess PCNA levels in hundreds of scientific studies.PCNA is mono-ubiquitinated at Lys164 in response to DNA damage at stalled replication forks. This modification is important in the translesion synthesis (TLS) DNA repair pathway (reviewed in 4,5).

1. Kelman, Z. and O'Donnell, M. (1995) Nucleic Acids Res 23, 3613-20.
2. Krishna, T.S. et al. (1994) Cell 79, 1233-43.
3. Maga, G. and Hubscher, U. (2003) J Cell Sci 116, 3051-60.
4. Lee, K.Y. and Myung, K. (2008) Mol Cells 26, 5-11.
5. Chen, J. et al. (2011) Cell Biochem Biophys 60, 47-60.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150
mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02%
sodium azide. Store at –20°C. Do not aliquot the antibody.