CYP3A4 (D9U6N) Rabbit mAb

• 产品编号：CST-13384S      品牌：CST       原厂货号：13384S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

CYP3A4 (D9U6N) Rabbit mAb兔单抗可以识别内源性CYP3A4总蛋白水平。单抗由合成肽段免疫动物获得，肽段与人CYP3A4蛋白的羧基端及其邻近氨基酸序列一致。细胞色素P450 3A亚家族多肽4（CYP3A4）是单加氧酶（1），被发现于肝脏和前列腺微粒体的内质网膜。这是一个对药物代谢十分重要的酶，催化目前市场中约50％的药物，类固醇，脂肪酸，和一些致癌物的第一阶段氧化反应（2）。它也参与了类固醇和胆固醇的合成（3,4）。 CYP3A4的表达可被糖皮质激素，致癌物质，杀虫剂和药物诱导，这可能会导致药物相互作用和中毒（5）。

1. Gupta, R.P. et al. (2004) J Bone Miner Res 19, 680-8.
2. Lynch, T. and Price, A. (2007) Am Fam Physician 76, 391-6.
3. Pikuleva, I.A. (2006) Pharmacol Ther 112, 761-73.
4. Tompkins, L.M. and Wallace, A.D. (2007) J Biochem Mol Toxicol 21, 176-81.
5. Thummel, K.E. and Wilkinson, G.R. (1998) Annu Rev Pharmacol Toxicol 38, 389-430.

原厂资料：

Specificity / Sensitivity

CYP3A4 (D9U6N) Rabbit mAb recognizes endogenous levels of total CYP3A4 protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human CYP3A4 protein.

Background

Cytochrome P450, subfamily 3A, polypeptide 4 (CYP3A4) is a mono-oxygenase enzyme (1) found in the endoplasmic reticulum membrane of liver and prostate microsomes. It is an important enzyme in drug metabolism; it catalyzes phase I oxidation reactions in approximately 50% of drugs in the modern market, as well as steroids, fatty acids, and some carcinogens (2). It is also involved in steroid and cholesterol synthesis (3,4). Expression of CYP3A4 can be induced by glucocorticoids, carcinogens, pesticides, and drugs, which can lead to drug interactions and toxicity (5).

1. Gupta, R.P. et al. (2004) J Bone Miner Res 19, 680-8.
2. Lynch, T. and Price, A. (2007) Am Fam Physician 76, 391-6.
3. Pikuleva, I.A. (2006) Pharmacol Ther 112, 761-73.
4. Tompkins, L.M. and Wallace, A.D. (2007) J Biochem Mol Toxicol 21, 176-81.
5. Thummel, K.E. and Wilkinson, G.R. (1998) Annu Rev Pharmacol Toxicol 38, 389-430.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150
mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02%
sodium azide. Store at –20°C. Do not aliquot the antibody.