TFIIE-α Antibody

• 产品编号：CST-13375S      品牌：CST       原厂货号：13375S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

TFIIE-α Antibody 兔多抗能够检测内源性TFIIE-α总蛋白。此多克隆抗体是采用合成的与人源TFIIE-α蛋白的Gly243残基周围序列相对应的肽段免疫动物而生产的。多克隆抗体由蛋白质A和多肽亲和层析纯化而成。

原厂资料：

Specificity / Sensitivity

TFIIE-α Antibody recognizes endogenous levels of total TFIIE-α protein.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly243 of human TFIIE-α protein. Antibodies are purified by protein A and peptide affinity chromatography.

Background

Transcription initiation factor IIE subunit alpha (TFIIE-α) is part of TFIIE, a general transcription factor made up of paired α and β subunits. These general transcription factors include TFIIA, TFIIB, TFIID, TFIIE, TFIIF, and TFIIH (1-4). Binding of RNA polymerase II (RNAPII) to promoter sequences as part of the pre-initiation complex (PIC) is facilitated by these general transcription factors. These factors also help in selection of the proper transcription start site, DNA unwinding, and RNAPII promoter escape during transcription (1). During the transition from transcription initiation to elongation, TFIIE stimulates the TFIIH kinase and DNA helicase activities, responsible for phosphorylation of the carboxy-terminal domain of the largest RNAPII subunit (POL2RA) and unwinding of promoter DNA for RNAPII promoter escape (1,5-9).

1. Thomas, M.C. and Chiang, C.M. (2006) Crit Rev Biochem Mol Biol 41, 105-78.
2. Ohkuma, Y. et al. (1991) Nature 354, 398-401.
3. Sumimoto, H. et al. (1991) Nature 354, 401-4.
4. Peterson, M.G. et al. (1991) Nature 354, 369-73.
5. Ohkuma, Y. and Roeder, R.G. (1994) Nature 368, 160-3.
6. Ohkuma, Y. et al. (1995) Mol Cell Biol 15, 4856-66.
7. Serizawa, H. et al. (1994) J Biol Chem 269, 20750-6.
8. Larochelle, S. et al. (2012) Nat Struct Mol Biol 19, 1108-15.
9. Grünberg, S. et al. (2012) Nat Struct Mol Biol 19, 788-96.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM
NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C.
Do not aliquot the antibody