Thioredoxin 2 Antibody

• 产品编号：CST-13322S      品牌：Cell Signaling Technology       原厂货号：13322S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

Thioredoxin 2可以识别内源性Thioredoxin 2总蛋白水平。该抗体可能和未知的60 kDa 到140 kDa的蛋白发生交叉反应。多抗由合成肽段免疫动物产生，该肽段与人thioredoxin 2蛋白羧基端肽段序列对应。抗体由蛋白A和肽段亲和层析技术纯化得到。硫氧还蛋白是一种小的氧化还原蛋白，在许多真核生物和原核生物中发现。一对在高度保守的活性位点序列中的半胱氨酸可被氧化形成二硫键，然后由硫氧还蛋白还原酶还原（1）。多种形式的硫氧还蛋白已经被确定，包括细胞内硫氧还蛋白1（Trx1）和线粒体硫氧还蛋白2（Trx2）。硫氧还蛋白参与许多细胞过程，包括氧化还原信号通路，氧化应激反应和蛋白质还原（1）。在许多正在研究的人类疾病如癌症，衰老和心脏疾病中，硫氧还蛋白可能具有潜在作用（2）。硫氧还蛋白可以在癌症的发展中发挥关键作用，因为它可以对促凋亡激酶ASK1进行负调节（3）。硫氧还蛋白表达的变化已与脑膜炎双球菌感染性休克和急性肺损伤相关联（4,5）。

1. Watson, W.H. et al. (2004) Toxicol Sci 78, 3-14.
2. Burke-Gaffney, A. et al. (2005) Trends Pharmacol Sci 26, 398-404.
3. Saitoh, M. et al. (1998) EMBO J 17, 2596-606.
4. Callister, M.E. et al. (2007) Intensive Care Med 33, 364-7.
5. Callister, M.E. et al. (2006) Thorax 61, 521-7.

原厂资料：

Specificity / Sensitivity

Thioredoxin 2 Antibody recognizes endogenous levels of total thioredoxin 2 protein. This antibody may cross-react with unidentified proteins from 60 kDa to 140 kDa.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human thioredoxin 2 protein. Antibodies are purified by protein A and peptide affinity chromatography.

Background

Thioredoxin is a small redox protein found in many eukaryotes and prokaryotes. A pair of cysteines within a highly conserved, active site sequence can be oxidized to form a disulfide bond that is then reduced by thioredoxin reductase (1). Multiple forms of thioredoxin have been identified, including cytosolic thioredoxin 1 (TRX1) and mitochondrial thioredoxin 2 (TRX2). Thioredoxin participates in many cellular processes including redox signaling, response to oxidative stress and protein reduction (1). A potential role of thioredoxin in human disorders such as cancer, aging and heart disease is currently under investigation (2). Thioredoxin can play a key role in cancer progression, as it acts as a negative regulator of the proapoptotic kinase ASK1 (3). Changes in thioredoxin expression have been associated with meningococcal septic shock and acute lung injury (4,5).

1. Watson, W.H. et al. (2004) Toxicol Sci 78, 3-14.
2. Burke-Gaffney, A. et al. (2005) Trends Pharmacol Sci 26, 398-404.
3. Saitoh, M. et al. (1998) EMBO J 17, 2596-606.
4. Callister, M.E. et al. (2007) Intensive Care Med 33, 364-7.
5. Callister, M.E. et al. (2006) Thorax 61, 521-7.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM
NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C.
Do not aliquot the antibody.