FoxP3 (D6O8R) Rabbit mAb?(Alexa Fluor? 488 Conjugate)

• 产品编号：CST-13282S      品牌：CST       原厂货号：13282S
• 产品分类：抗体 > 一抗 > 染料标记抗体
• 应用分类：

描述：

Specificity / Sensitivity

FoxP3 (D6O8R) XP^® Rabbit mAb (Alexa Fluor^® 488 Conjugate) recognizes endogenous levels of total FoxP3 protein. This antibody recognizes both human and mouse FoxP3 protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro44 of mouse FoxP3 protein.

Description

This Cell Signaling Technology antibody is conjugated to Alexa Fluor^® 488 fluorescent dye and tested in-house for direct flow cytometry in mouse cells. The antibody is expected to exhibit the same species cross-reactivity as the unconjugated FoxP3 (D6O8R) XP^® Rabbit mAb #12653.

原厂资料：

Specificity / Sensitivity

FoxP3 (D6O8R) XP^® Rabbit mAb (Alexa Fluor^® 488 Conjugate) recognizes endogenous levels of total FoxP3 protein. This antibody recognizes both human and mouse FoxP3 protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro44 of mouse FoxP3 protein.

Description

This Cell Signaling Technology antibody is conjugated to Alexa Fluor^® 488 fluorescent dye and tested in-house for direct flow cytometry in mouse cells. The antibody is expected to exhibit the same species cross-reactivity as the unconjugated FoxP3 (D6O8R) XP^® Rabbit mAb #12653.

Background

Forkhead box (Fox) proteins are a family of evolutionarily conserved transcription factors containing a sequence known as Forkhead box or winged helix DNA binding domain (1). The human genome contains 43 Fox proteins that are divided into subfamilies. The FoxP subfamily has four members, FoxP1 - FoxP4, which are broadly expressed and play important roles in organ development, immune response and cancer pathogenesis (2-4). The FoxP subfamily has several characteristics that are atypical among Fox proteins: their Forkhead domain is located at the carboxy-terminal region and they contain motifs that promote homo- and heterodimerization. FoxP proteins usually function as transcriptional repressors (4,5).

FoxP3 is crucial for the development of T cells with regulatory properties (Treg) (6). Mutations in FoxP3 are associated with immune dysregulation, polyendocrinopathy, enteropathy, and X-linked syndrome (IPEX) (7), while overexpression in mice causes severe immunodeficiency (8). Research studies have shown that FoxP3 functions as a tumor suppressor in several types of cancer (9-11).

1. Myatt, S.S. and Lam, E.W. (2007) Nat Rev Cancer 7, 847-59.
2. Shu, W. et al. (2001) J Biol Chem 276, 27488-97.
3. Lu, M.M. et al. (2002) Gene Expr Patterns 2, 223-8.
4. Koon, H.B. et al. (2007) Expert Opin Ther Targets 11, 955-65.
5. Li, S. et al. (2004) Mol Cell Biol 24, 809-22.
6. Ochs, H.D. et al. (2007) Immunol Res 38, 112-21.
7. Bennett, C.L. et al. (2001) Nat Genet 27, 20-1.
8. Kasprowicz, D.J. et al. (2003) J Immunol 171, 1216-23.
9. Zuo, T. et al. (2007) Cell 129, 1275-86.
10. Zuo, T. et al. (2007) J Clin Invest 117, 3765-73.
11. Wang, L. et al. (2009) Cancer Cell 16, 336-46.

注意事项：

Storage: Supplied in PBS (pH 7.2), less than 0.1% sodium
azide and 2 mg/ml BSA. Store at 4°C. Do not aliquot the
antibody. Protect from light. Do not freeze.