BAP1 (D7W7O) Rabbit mAb

• 产品编号：CST-13271S      品牌：CST       原厂货号：13271S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

BAP1 (D7W7O) Rabbit mAb兔单抗识别内源性的BAP1总蛋白。此单克隆抗体经合成与人BRCA1的Leu709邻近氨基酸残基序列一致的肽段，免疫动物获得。AP1(BRCA1相关蛋白1)最初鉴定为一个与BRCA1相偶联的并定位于核的泛素水解酶，具有抑制细胞增殖功能。此蛋白属于UCH去泛素化酶家族成员，在N端含有UCH结构域，在C端含有BRCA1相互作用结构域和核定位信号序列(1)。在肺癌和乳腺癌标本中常发现 BAP1基因的频繁重组和缺失(1,2)，在肿瘤细胞系和动物癌变模型中进行体内突变分析发现，其去泛素化活性和核定位信号对于其发挥肿瘤抑制作用比较重要。BAP1并不直接对自泛素化的BRCA1/BARD1 E3复合体发挥去泛素化功能(4)，但其与BARD1相互作用并阻碍复合体的二聚化过程，从而抑制 BRCA1/BARD1 E3复合体活性(5)。除上述作用外, BAP1还与HCF-1相互作用并去泛素化，从而控制HCF-1的稳定性(6).

1. Jensen, D.E. et al. (1998) Oncogene 16, 1097-112.
2. Buchhagen, D.L. et al. (1994) Int J Cancer 57, 473-9.
3. Ventii, K.H. et al. (2008) Cancer Res 68, 6953-62.
4. Mallery, D.L. et al. (2002) EMBO J 21, 6755-62.
5. Nishikawa, H. et al. (2009) Cancer Res 69, 111-9.
6. Misaghi, S. et al. (2009) Mol Cell Biol 29, 2181-92.

原厂资料：

Specificity / Sensitivity

BAP1 (D7W7O) Rabbit mAb recognizes endogenous levels of total BAP1 protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Leu709 within the BRCA1 interaction region of human BAP1 protein.

Background

BRCA1-Associated Protein 1 (BAP1) was originally identified as a BRCA1 associated, nuclear localized ubiquitin hydrolase that suppresses cell growth (1). The protein belongs to the UCH family of deubiquitinases, with a UCH domain in its amino-terminal segment and a BRCA1 interaction domain as well as a nuclear localization signal in its carboxy-terminal segment (1). Frequent gene locus rearrangement, deletion, and null mutation of BAP1 have been found in lung and breast cancers (1,2). In vivo mutation analysis of cancer cell line survival and animal tumorigenesis indicates that both the deubiquitinase activity and the nuclear localization signal are required for BAP1 function as a tumor suppressor (3). BAP1 does not have direct deubiquitination activity towards the autoubiquitinated BRCA1/BARD1 E3 complex (4), but its interaction with BARD1 inhibits BRCA1/BARD1 E3 activity by interfering with the complex dimerization process (5). In addition to its interaction with BRCA1/BARD1, BAP1 has also been shown to interact with and deubiquitinate HCF-1, thereby controlling its stability (6).

1. Jensen, D.E. et al. (1998) Oncogene 16, 1097-112.
2. Buchhagen, D.L. et al. (1994) Int J Cancer 57, 473-9.
3. Ventii, K.H. et al. (2008) Cancer Res 68, 6953-62.
4. Mallery, D.L. et al. (2002) EMBO J 21, 6755-62.
5. Nishikawa, H. et al. (2009) Cancer Res 69, 111-9.
6. Misaghi, S. et al. (2009) Mol Cell Biol 29, 2181-92.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150
mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02%
sodium azide. Store at –20°C. Do not aliquot the antibody.