IGFBP3 Antibody

• 产品编号：CST-13216S      品牌：CST       原厂货号：13216S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

IGFBP3 Antibody抗体可以检测内源性IGFBP3总蛋白水平。多抗由合成肽段免疫动物产生，该肽段与人IGFBP3蛋白Ser155邻近氨基酸序列一致。抗体由蛋白A和肽段亲和层析技术纯化得到胰岛素样生长因子(IGF)信号通路在调控正常和癌细胞增殖和代谢过程中发挥了主要作用(1)。胰岛素样生长因子结合蛋白(IGFBPs)在多种细胞中调控IGF活动发挥了了不可或缺的作用。两个IGFBP家族成员都和IGF有高度亲和力，并且结构相关却又由不同的基因编码(1)。IGF结合蛋白可以通过羧基端和IGF的高亲结合控制IGF的可用性而施加刺激或抑制作用(2,3)。IGFBP3是最丰富的血清IGF结合蛋白，以及IGF- I生物活性的主要介导因素。IGFBP3也结合IGF-II, insulin,和其他细胞内外成分，通过IGF依赖和非依赖机制，调控细胞生长，发育，和凋亡(4-8)。研究表明IGF- I水平增加，IGFBP3水平降低会导致患癌风险增加，包括乳腺癌，结肠癌，肺癌和前列腺癌(2)。

1. Hwa, V. et al. (1999) Endocr Rev 20, 761-87.
2. Yu, H. and Rohan, T. (2000) J Natl Cancer Inst 92, 1472-89.
3. Martin, J.L. and Baxter, R.C. (2011) Growth Factors 29, 235-44.
4. Zapf, J. et al. (1990) J Biol Chem 265, 14892-8.
5. Coverley, J.A. and Baxter, R.C. (1997) Mol Cell Endocrinol 128, 1-5.
6. Ingermann, A.R. et al. (2010) J Biol Chem 285, 30233-46.
7. Liu, B. et al. (2000) J Biol Chem 275, 33607-13.
8. Baxter, R.C. (2001) Mol Pathol 54, 145-8.

原厂资料：

Specificity / Sensitivity

IGFBP3 Antibody recognizes endogenous levels of total IGFBP3 protein.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ser155 of human IGFBP3 protein. Antibodies are purified by protein A and peptide affinity chromatography.

Background

Insulin-like growth factor (IGF) signaling plays a major role in regulating the proliferation and metabolism of normal and malignant cells (1). Insulin-like growth factor-binding proteins (IGFBPs) play an integral role in modifying IGF actions in a wide variety of cell types. The six IGFBP family members share a high affinity for IGF binding and are structurally related, but are encoded by distinct genes (1). IGF binding proteins can exert stimulatory or inhibitory effects by controlling IGF availability through high affinity binding of IGF at the carboxy-terminal domain (2,3). IGFBP3 is the most abundant serum IGF binding protein and the main mediator for IGF-I bioactivities. IGFBP3 also binds IGF-II, insulin, and other cellular and extracellular components to regulate cell growth, development, and apoptosis through both IGF-dependent and IGF-independent mechanisms (4-8). Research studies describe correlations between increased IGF-I levels and reduced levels of IGFBP3 with increased risks of developing cancer, including breast, colon, lung, and prostate cancer (2).

1. Hwa, V. et al. (1999) Endocr Rev 20, 761-87.
2. Yu, H. and Rohan, T. (2000) J Natl Cancer Inst 92, 1472-89.
3. Martin, J.L. and Baxter, R.C. (2011) Growth Factors 29, 235-44.
4. Zapf, J. et al. (1990) J Biol Chem 265, 14892-8.
5. Coverley, J.A. and Baxter, R.C. (1997) Mol Cell Endocrinol 128, 1-5.
6. Ingermann, A.R. et al. (2010) J Biol Chem 285, 30233-46.
7. Liu, B. et al. (2000) J Biol Chem 275, 33607-13.
8. Baxter, R.C. (2001) Mol Pathol 54, 145-8.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM
NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C.
Do not aliquot the antibody.