Golgin-97 (D8P2K) Rabbit mAb

• 产品编号：CST-13192S      品牌：CST       原厂货号：13192S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

</p>Golgin-97 (D8P2K) Rabbit mAb可以识别内源性总的golgin-97蛋白。</p>单克隆抗体由合成磷酸肽段免疫动物产生，该肽段与人golgin-97蛋白Leu663附近氨基酸一致。</p>高尔基体相关蛋白golgin A1 (GOLGA1, golgin-97)最早被认为是一个高尔基体复合物自身抗原，和自身免疫综合征Sjogren's疾病相关（1）。golgin-97有一个羧基端GRIP结构域，经常被作为trans-Golgi network (TGN)的靶标。所有已知的四个哺乳动物带GRIP结构域蛋白(golgin-97, golgin-245, GCC88 and GCC185)都在TGN被发现，共享了alpha-螺旋结构并形成了同二聚体（2）。当所有的四个golgin蛋白都定位到TGN后，它们占线了不同的细胞膜结合能力，并被发现在不同的TGN区域（3）。Golgin-97 和 golgin-245通过它们的GRIP结构域和Arl1蛋白switch II相互作用而定位在trans-Golgi (4)。对含有GRIP结构域的蛋白进行过表达和敲除实验发现这些蛋白帮助维持了trans-Golgi网络的一体性，并通过控制TGN残留蛋白的位置而发挥功能（5）。通过使用Shiga toxin B fragment (STxB)-based体外运输实验和E-cadherin转运模型体系，结果表明golgin-97和它的效应分子Arl1-GTP在trans-Golgi内体运输过程中发挥了作用（6,7）。研究也表明golgin-97可能参与了痘病毒的生产和成束（8,9）。

1. Griffith, K.J. et al. (1997) Arthritis Rheum 40, 1693-702.
2. Luke, M.R. et al. (2005) Biochem J 388, 835-41.
3. Derby, M.C. et al. (2004) J Cell Sci 117, 5865-74.
4. Lu, L. and Hong, W. (2003) Mol Biol Cell 14, 3767-81.
5. Yoshino, A. et al. (2003) J Cell Sci 116, 4441-54.
6. Lu, L. et al. (2004) Mol Biol Cell 15, 4426-43.
7. Lock, J.G. et al. (2005) Traffic 6, 1142-56.
8. Alzhanova, D. and Hruby, D.E. (2006) J Virol 80, 11520-7.
9. Alzhanova, D. and Hruby, D.E. (2007) Virology 362, 421-7.

原厂资料：

Specificity / Sensitivity

<p>Golgin-97 (D8P2K) Rabbit mAb recognizes endogenous levels of total golgin-97 protein.

Source / Purification

<p>Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Leu663 of human golgin-97 protein.</

Background

<p>The Golgi-associated protein golgin A1 (GOLGA1, golgin-97) was first isolated as a Golgi complex autoantigen associated with the autoimmune disorder Sjogren's syndrome (1). The golgin-97 protein contains a carboxy-terminal GRIP domain and is a commonly used trans-Golgi network (TGN) marker. All four known mammalian GRIP domain-containing proteins (golgin-97, golgin-245, GCC88 and GCC185) are found in the TGN, share extensive alpha-helical structure, and form homodimers (2). While all four golgin proteins localize to the TGN, they exhibit different membrane-binding abilities and are found in distinct TGN regions (3). Golgin-97 and golgin-245 are targeted to the trans-Golgi network through an interaction between their GRIP domains and the Arl1 protein switch II region (4). Overexpression studies and siRNA assays with GRIP domain-containing proteins suggest that these proteins help to maintain trans-Golgi network integrity and function by controlling localization of TGN resident proteins (5). By using a Shiga toxin B fragment (STxB)-based in vitro transport assay and an E-cadherin transport model system, golgin-97 and its effector Arl1-GTP were shown to play a role in trans-Golgi endosomal trafficking (6,7). Research studies also suggest that golgin-97 may play a role in poxvirus morphogenesis and maturation (8,9).

1. Griffith, K.J. et al. (1997) Arthritis Rheum 40, 1693-702.
2. Luke, M.R. et al. (2005) Biochem J 388, 835-41.
3. Derby, M.C. et al. (2004) J Cell Sci 117, 5865-74.
4. Lu, L. and Hong, W. (2003) Mol Biol Cell 14, 3767-81.
5. Yoshino, A. et al. (2003) J Cell Sci 116, 4441-54.
6. Lu, L. et al. (2004) Mol Biol Cell 15, 4426-43.
7. Lock, J.G. et al. (2005) Traffic 6, 1142-56.
8. Alzhanova, D. and Hruby, D.E. (2006) J Virol 80, 11520-7.
9. Alzhanova, D. and Hruby, D.E. (2007) Virology 362, 421-7.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150
mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02%
sodium azide. Store at –20°C. Do not aliquot the antibody.