Calbindin (D1I4Q) XP? Rabbit mAb

• 产品编号：CST-13176P      品牌：Cell Signaling Technology       原厂货号：13176P
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

Calbindin (D1I4Q) XP® Rabbit mAb 兔单抗可以识别内源性的calbindin总蛋白。该单克隆抗体由重组蛋白免疫动物产生，该肽段与人calbindin蛋白氨基末端附近氨基酸一致。不同亚家族的钙结合蛋白调节第二信使钙。钙结合蛋白，钙调蛋白，S-100，小白蛋白（parvalbumin，PV）和肌钙蛋白C都是低分子量钙结合蛋白家族的成员（1）。钙结合蛋白在中枢神经系统内的部分离散的神经集群中表达，被认为是细胞内的钙离子缓冲蛋白。大多数浦肯野细胞在神经元开始迁移和分化时表达钙结合蛋白。相比之下，其它钙离子缓冲蛋白，如小白蛋白，是在其后的生长过程中随着神经元活动的增加而增加（2）。

1. Kawasaki, H. et al. (1998) Biometals 11, 277-95.
2. Bastianelli, E. (2003) Cerebellum 2, 242-62.

原厂资料：

Specificity / Sensitivity

Calbindin (D1I4Q) XP^® Rabbit mAb recognizes endogenous levels of total calbindin protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with recombinant protein specific to the amino terminus of human calbindin protein.

Background

Calcium-binding proteins of different subfamilies regulate the second messenger calcium. Calbindin, calmodulin, S-100, parvalbumin and troponin C are members of the low molecular weight calcium-binding protein family (1). Calbindin is expressed in discrete neuronal populations within the CNS and is thought to act as an intracellular calcium buffering protein. Most Purkinje cells express calbindin, which is expressed when neurons start to migrate and differentiate. In contrast, other calcium buffering proteins, such as parvalbumin, are expressed later during development and in parallel with increasing neuronal activity (2).

1. Kawasaki, H. et al. (1998) Biometals 11, 277-95.
2. Bastianelli, E. (2003) Cerebellum 2, 242-62.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150
mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02%
sodium azide. Store at –20°C. Do not aliquot the antibody.