NT5E/CD73 (D7F9A) Rabbit mAb

• 产品编号：CST-13160S      品牌：CST       原厂货号：13160S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

NT5E/CD73 (D7F9A) Rabbit mAb 兔单抗可以识别内源性的NT5E/CD73总蛋白。该单克隆抗体由人NT5E/CD73蛋白氨基末端重组蛋白免疫动物产生。Ecto-5'-nucleotidase (NT5E, 也被称为CD73)是一个70kDa的糖基磷脂酰肌醇锚定的膜结合糖蛋白，其催化细胞外单磷酸核苷水解成有生物活性的核苷(1)。NT5E催化细胞外磷酸腺苷形成腺苷，从而推动细胞外腺苷水平的调节和四个G蛋白偶联腺苷受体的下游信号激活(2)。缺氧诱导因子(HIF-1)和NT5E基因启动子结合导致缺氧时NT5E表达上调(3)。NT5E的生物功能包括林把细胞黏附(4,5)，纤维化(6)和伤害感受的调节(7,8)。

1. Le Hir, M. and Kaissling, B. (1993) Am J Physiol 264, F377-87.
2. Schulte, G. and Fredholm, B.B. (2003) Cell Signal 15, 813-27.
3. Synnestvedt, K. et al. (2002) J Clin Invest 110, 993-1002.
4. Arvilommi, A.M. et al. (1997) Eur J Immunol 27, 248-54.
5. Airas, L. and Jalkanen, S. (1996) Blood 88, 1755-64.
6. Peng, Z. et al. (2008) Nucleosides Nucleotides Nucleic Acids 27, 821-4.
7. Sowa, N.A. et al. (2010) J Neurosci 30, 2235-44.
8. Zylka, M.J. (2011) Trends Mol Med 17, 188-96.

原厂资料：

Specificity / Sensitivity

NT5E/CD73 (D7F9A) Rabbit mAb recognizes endogenous levels of total NT5E/CD73 protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with recombinant protein specific to the amino terminus of human NT5E/CD73 protein.

Background

Ecto-5'-nucleotidase (NT5E, also called CD73) is a 70 kDa glycosyl phosphatidylinositol-anchored, membrane-bound glycoprotein that catalyzes the hydrolysis of extracellular nucleoside monophosphates into bioactive nucleosides (1). NT5E catalyzes the terminal step of extracellular adenosine formation from adenosine monophosphate, which drives the regulation of extracellular adenosine levels and the downstream activation of the four G protein-coupled adenosine receptors (2). Binding of hypoxia-inducible factor (HIF-1) to the NT5E gene promoter leads to upregulation of NT5E during hypoxia (3). The biological roles of NT5E include lymphocyte adhesion (4,5), fibrosis (6), and the regulation of nociception (7,8).

1. Le Hir, M. and Kaissling, B. (1993) Am J Physiol 264, F377-87.
2. Schulte, G. and Fredholm, B.B. (2003) Cell Signal 15, 813-27.
3. Synnestvedt, K. et al. (2002) J Clin Invest 110, 993-1002.
4. Arvilommi, A.M. et al. (1997) Eur J Immunol 27, 248-54.
5. Airas, L. and Jalkanen, S. (1996) Blood 88, 1755-64.
6. Peng, Z. et al. (2008) Nucleosides Nucleotides Nucleic Acids 27, 821-4.
7. Sowa, N.A. et al. (2010) J Neurosci 30, 2235-44.
8. Zylka, M.J. (2011) Trends Mol Med 17, 188-96.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150
mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02%
sodium azide. Store at –20°C. Do not aliquot the antibody.