TAF15 Antibody

• 产品编号：CST-13150S      品牌：Cell Signaling Technology       原厂货号：13150S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

TAF15 Antibody 兔多抗能够检测内源性TAF15总蛋白。多克隆抗体是采用合成的与人源 TAF15 蛋白的近羧基末端的多肽免疫动物而生产的。由蛋白质A和多肽亲和层析纯化而成。TBP binding protein associated factor 15 (TAF15)是多功能性FET (FUS, EWS, 以及TAF15)蛋白家族的成员之一，该蛋白家族能与DNA 和 RNA结合，可能对于转录调控以及RNA加工都非常重要（1,2）。研究发现FET家族蛋白，包括TAF15，再许多癌症中，可作为转录因子的肿瘤发生融合蛋白。该融合蛋白由FET蛋白氨基末端转录激活结构域和转录因子DNA结合结构域组成，产生一个畸变的具有有效转录激活潜能的转录因子（1-3）。TAF15可作为transcription factor IID (TFIID) complex的一个子集的一部分，这意味着它可能能够在活化基因上形成一个转录起始前复合物，即使它并不被认为是一个规范的TAF（4,5）。还有一些研究表明，TAF15可以通过其与U1 small nuclear RNA的结合在RNA剪接中发挥作用，并且还能通过一种参与miRNA生物合成的机制来调节细胞周期调控基因的表达（6,7）。

1. Law, W.J. et al. (2006) Brief Funct Genomic Proteomic 5, 8-14.
2. Kovar, H. (2011) Sarcoma 2011, 837474.
3. Bertolotti, A. et al. (1999) Oncogene 18, 8000-10.
4. Bertolotti, A. et al. (1996) EMBO J 15, 5022-31.
5. Bertolotti, A. et al. (1998) Mol Cell Biol 18, 1489-97.
6. Jobert, L. et al. (2009) EMBO Rep 10, 494-500.
7. Ballarino, M. et al. (2012) Oncogene , (Epub ahead of print).

原厂资料：

Homology

Species predicted to react based on 100% sequence homology: Hamster, Bovine, Dog, Pig, Horse

Specificity / Sensitivity

TAF15 Antibody recognizes endogenous levels of total TAF15 protein.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human TAF15 protein. Antibodies are purified by protein A and peptide affinity chromatography.

Background

TBP binding protein associated factor 15 (TAF15) is a member of the multifunctional FET (FUS, EWS, and TAF15) family of proteins that bind both DNA and RNA and may be important for transcriptional regulation and RNA processing (1,2). Research studies show that FET family proteins, including TAF15, can be found as oncogenic fusion proteins with transcription factors in a variety of cancers. The fusion protein consists of the FET protein amino-terminal transcription activation domain and the transcription factor DNA binding domain, creating an aberrant transcription factor with potent transactivation potential (1-3). TAF15 can be part of a subset of the transcription factor IID (TFIID) complex, indicating that it may form a transcription pre-initiation complex at active genes, even if it is not considered a canonical TAF (4,5). Additional studies suggest roles for TAF15 in RNA splicing through its association with U1 small nuclear RNA and in regulation of cell cycle regulatory gene expression through a mechanism involving miRNA biosynthesis (6,7).

1. Law, W.J. et al. (2006) Brief Funct Genomic Proteomic 5, 8-14.
2. Kovar, H. (2011) Sarcoma 2011, 837474.
3. Bertolotti, A. et al. (1999) Oncogene 18, 8000-10.
4. Bertolotti, A. et al. (1996) EMBO J 15, 5022-31.
5. Bertolotti, A. et al. (1998) Mol Cell Biol 18, 1489-97.
6. Jobert, L. et al. (2009) EMBO Rep 10, 494-500.
7. Ballarino, M. et al. (2012) Oncogene , (Epub ahead of print).

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM
NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C.
Do not aliquot the antibody