TRF2 (D1Y5D) Rabbit mAb

• 产品编号：CST-13136S      品牌：CST       原厂货号：13136S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

Trf2 (D1Y5D) Rabbit mAb可以识别内源性总的Trf2蛋白。单克隆抗体由合成重组人Trf2蛋白免疫动物产生。端粒，染色体的线性末端，折叠成T-loops以避免被细胞识别为DNA双链损伤(DSBs) (1)。端粒重复结合因子蛋白Trf1 和 Trf2与双链端粒结合以形成T-loops（2）。已经发现大量涉及DNA损伤反应的蛋白与Trf2在端粒区域结合（3）。有意思的是，Trf2可以短时间的位于DNA损伤诱导的DSBs，但是过表达Trf2会阻止ATM-依赖的信号通路（4）。体内发现了Trf2的Ser323磷酸化，但是没有发现该磷酸位点的上游激酶以及功能（50。

1. Karlseder, J. et al. (1999) Science 283, 1321-5.
2. Yoshimura, S.H. et al. (2004) Genes Cells 9, 205-18.
3. Colgin, L. and Reddel, R. (2004) Curr Biol 14, R901-2.
4. Bradshaw, P.S. et al. (2005) Nat Genet 37, 193-7.
5. Beausoleil, S.A. et al. (2004) Proc Natl Acad Sci U S A 101, 12130-5.

原厂资料：

Specificity / Sensitivity

Trf2 (D1Y5D) Rabbit mAb recognizes endogenous levels of total Trf2 protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with recombinant protein specific to human Trf2 protein.

Background

Telomeres, the linear ends of chromosomes, are organized into T-loops to prevent them from being recognized by the cell as DNA double stranded breaks (DSBs) (1). The telomeric repeat binding factor proteins Trf1 and Trf2 bind to double-stranded telomeres to allow formation of T-loops (2). A large number of proteins involved in the DNA damage response are found physically associated with Trf2 within telomeres (3). Interestingly, Trf2 can transiently localize to DNA damage-induced DSBs, but overexpression of Trf2 prevents ATM-dependent signaling (4). Phosphorylation of Trf2 at Ser323 has been reported in vivo, but no upstream kinase or role has been established for this phosphorylation site (5).

1. Karlseder, J. et al. (1999) Science 283, 1321-5.
2. Yoshimura, S.H. et al. (2004) Genes Cells 9, 205-18.
3. Colgin, L. and Reddel, R. (2004) Curr Biol 14, R901-2.
4. Bradshaw, P.S. et al. (2005) Nat Genet 37, 193-7.
5. Beausoleil, S.A. et al. (2004) Proc Natl Acad Sci U S A 101, 12130-5.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150
mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02%
sodium azide. Store at –20°C. Do not aliquot the antibody.