IFITM1 Antibody

• 产品编号：CST-13126S      品牌：CST       原厂货号：13126S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

Specificity / Sensitivity

IFITM1 Antibody recognizes endogenous levels of total IFITM1 protein. This antibody does not cross-react with IFITM2 or IFITM3 proteins.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro20 of human IFITM1 protein. Antibodies are purified by protein A and peptide affinity chromatography.

原厂资料：

Specificity / Sensitivity

IFITM1 Antibody recognizes endogenous levels of total IFITM1 protein. This antibody does not cross-react with IFITM2 or IFITM3 proteins.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro20 of human IFITM1 protein. Antibodies are purified by protein A and peptide affinity chromatography.

Background

Interferon-induced transmembrane protein (IFITM) family members are composed of short amino- and carboxy-termini, two transmembrane domains, and a cytoplasmic domain (1). There are four family members in humans: IFITM1, IFITM2, IFITM3, and IFITM5 (2,3). Mice have two additional family members, IFITM6 and IFITM7 (2,3). Basal expression of IFITM proteins is observed in some cells and expression can also be induced by type I and type II interferons (4-6). The primary function of IFITM family proteins appears to be viral restriction, as IFITM proteins inhibit cytosolic entry of viruses by preventing fusion of viral and host membranes (7,8). The mechanism by which IFITM proteins inhibit fusion is unclear. Although IFITM proteins are present on both the plasma membrane and intracellular membranes, they most effectively restrict viral fusion in late endosomes and lysosomes (8,9). In addition, different family members exhibit specific viral preferences (9). For example, IFITM3 is most effective at restricting influenza A infection, while IFITM1 is more successful in controlling filoviruses and SARS (9,10).

1. Diamond, M.S. and Farzan, M. (2013) Nat Rev Immunol 13, 46-57.
2. Lange, U.C. et al. (2003) BMC Dev Biol 3, 1.
3. Hickford, D. et al. (2012) BMC Genomics 13, 155.
4. Reid, L.E. et al. (1989) Proc Natl Acad Sci U S A 86, 840-4.
5. Lewin, A.R. et al. (1991) Eur J Biochem 199, 417-23.
6. Friedman, R.L. et al. (1984) Cell 38, 745-55.
7. Brass, A.L. et al. (2009) Cell 139, 1243-54.
8. Feeley, E.M. et al. (2011) PLoS Pathog 7, e1002337.
9. Huang, I.C. et al. (2011) PLoS Pathog 7, e1001258.
10. Everitt, A.R. et al. (2012) Nature 484, 519-23.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM
NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C.
Do not aliquot the antibody.