CD71 (D7G9X) XP? Rabbit mAb

• 产品编号：CST-13113P      品牌：Cell Signaling Technology       原厂货号：13113P
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

</p>单克隆抗体由合成磷酸肽段免疫动物产生，该肽段与人CD71蛋白Leu146附近氨基酸一致。</p>转铁蛋白受体1（CD71，TFRC）是一个II型跨膜受体和载体蛋白负责细胞通过受体介导的内吞作用摄取铁(1).细胞表面的种型pH促进铁结合糖蛋白转铁蛋白（Tf）和CD71受体结合。这个受体-配体复合物通过受体介导的内吞作用进入细胞，随后进入核内体。核内体相对较低的pH会导致转铁蛋白结合位点附近的电流发生改变而导致铁被释放（2）。这个受体配体复合物可以在细胞表面回收，在这里转铁蛋白会从CD71受体上脱离（2）。广泛表达的转铁蛋白受体可以持续回收并且无论配体在哪种结合状态都进入clathrin接到的内吞过程。这种受体配体的相互作用已经被仔细研究了。CD71的螺旋结构与可以直接和转铁蛋白C-lobe结合，并导致Tf的结构发生改变促进其转运过程（3）。受体CD71和转铁蛋白的相互作用是通过细胞膜蛋白hemochromatosis(HFE)介导的。HFE与CD71的α-螺旋结构域结合，阻止CD71-转铁蛋白复合物形成并抑制铁被吸收（4,5）。除了结合转铁蛋白，CD71也和细胞表面的H-ferritin结合，将这个细胞内的储铁蛋白运输到核内体和溶酶体（6）。更多的研究表明转铁蛋白受体是一个在进化上保守的受体，来自于多种沙粒病毒或至少一种逆转录病毒（7,8）。多种癌症中都发现了CD71的异常表达，包括甲状腺癌，淋巴瘤，和T-谱系白血病，提示通过抑制转铁蛋白可能作为一种治疗靶标(9,10)。

治疗靶标(9,10)。

1. Ponka, P. and Lok, C.N. (1999) Int J Biochem Cell Biol 31, 1111-37.
2. Bali, P.K. et al. (1991) Biochemistry 30, 324-8.
3. Cheng, Y. et al. (2004) Cell 116, 565-76.
4. Bennett, M.J. et al. (2000) Nature 403, 46-53.
5. Feder, J.N. et al. (1998) Proc Natl Acad Sci U S A 95, 1472-7.
6. Li, L. et al. (2010) Proc Natl Acad Sci U S A 107, 3505-10.
7. Radoshitzky, S.R. et al. (2007) Nature 446, 92-6.
8. Coffin, J.M. (2013) PLoS Biol 11, e1001574.
9. Magro, G. et al. (2011) Thyroid 21, 267-77.
10. Rodríguez, J.A. et al. (2011) Leuk Lymphoma 52, 2169-78.
11. Beardmore, J. et al. (1987) J Cell Sci 87 ( Pt 4), 495-506.
12. Zuk, P.A. and Elferink, L.A. (2000) J Biol Chem 275, 26754-64.

原厂资料：

Specificity / Sensitivity

<p>CD71 (D7G9X) XP^® Rabbit mAb recognizes endogenous levels of total CD71 protein.

Source / Purification

<p>Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Leu146 of human CD71 protein.

Background

<p>Transferrin receptor 1 (CD71, TFRC) is a type II transmembrane receptor and carrier protein responsible for the uptake of cellular iron through receptor-mediated endocytosis (1). Neutral pH at the cell surface promotes binding of the iron-binding glycoprotein transferrin (Tf) to the CD71 receptor. The receptor-ligand complex enters the cell through receptor-mediated endocytosis and is internalized into an endosome. Relatively lower endosomal pH leads to a change in the local charge environment surrounding the iron-transferrin binding site and results in the release of iron (2). The receptor-ligand complex is recycled to the cell surface where transferrin dissociates from the CD71 receptor (2). Ubiquitously expressed transferrin receptor is continuously recycled and undergoes clathrin-mediated endocytosis regardless of ligand binding state. The interaction between receptor and ligand has been studied in detail. The helical domain of CD71 directly interacts with the transferrin C-lobe and induces a conformation change in Tf to facilitate the transport process (3). Interaction between the receptor CD71 and transferrin is mediated by the membrane protein hemochromatosis (HFE). HFE binds the α-helical domain of CD71, blocking formation of the CD71-transferrin complex and inhibiting iron uptake (4,5). In addition to binding transferrin, CD71 also interacts with H-ferritin at the cell surface and transports this intracellular iron storage protein to cellular endosomes and lysosomes (6). Additional studies indicate that the transferrin receptor is an evolutionarily conserved receptor for a number or arenaviruses and at least one retrovirus (7,8). Aberrant expression of CD71 is seen in a number of cancers, including thyroid carcinomas, lymphomas, and T-lineage leukemias, suggesting a possible therapeutic role for targeted inhibition of the transferrin receptor (9,10).

治疗靶标(9,10)。

1. Ponka, P. and Lok, C.N. (1999) Int J Biochem Cell Biol 31, 1111-37.
2. Bali, P.K. et al. (1991) Biochemistry 30, 324-8.
3. Cheng, Y. et al. (2004) Cell 116, 565-76.
4. Bennett, M.J. et al. (2000) Nature 403, 46-53.
5. Feder, J.N. et al. (1998) Proc Natl Acad Sci U S A 95, 1472-7.
6. Li, L. et al. (2010) Proc Natl Acad Sci U S A 107, 3505-10.
7. Radoshitzky, S.R. et al. (2007) Nature 446, 92-6.
8. Coffin, J.M. (2013) PLoS Biol 11, e1001574.
9. Magro, G. et al. (2011) Thyroid 21, 267-77.
10. Rodríguez, J.A. et al. (2011) Leuk Lymphoma 52, 2169-78.
11. Beardmore, J. et al. (1987) J Cell Sci 87 ( Pt 4), 495-506.
12. Zuk, P.A. and Elferink, L.A. (2000) J Biol Chem 275, 26754-64.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150
mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02%
sodium azide. Store at –20°C. Do not aliquot the antibody