Phospho-CDCP1 (Tyr707)?Antibody

• 产品编号：CST-13111S      品牌：CST       原厂货号：13111S
• 产品分类：抗体 > 一抗 > 磷酸化抗体
• 应用分类：

描述：

Phospho-CDCP1 (Tyr707) Antibody可以识别Tyr707被磷酸化的内源性CDCP1蛋白。该抗体与Tyr734, Tyr743,或Tyr806磷酸化的CDCP1没有交叉反应，但是可能与其他酪氨酸被磷酸化的蛋白发生交叉反应。Cub1抗体处理ZR-75-1细胞提取物后western blot的结果会有一条80kDa不知源性的蛋白条带。多克隆抗体由合成肽段免疫动物产生，该合成肽段与人CDCP1蛋白的Tyr707附近残基相一致，抗体由肽段亲和层析技术纯化。CUB结构域包含蛋白1 (CDCP1, SIMA135)是研究中发现的潜在的干细胞标记，在某些人癌细胞和典型及非典型（癌性）结肠的结肠组织中都高表达(1)。CDCP1可以通过表观遗传学调控，启动子的CpG序列甲基化会降低CDCP1表达水平(2)。CDCp1基因编码的这个糖蛋白是一个复杂的，多结构域跨膜抗原。CDCP1有三个细胞外CUB结构域参与了细胞黏附和细胞外基质相互作用(1-3)。Src家族激酶可以磷酸化CDCP1的胞内结构域上五个酪氨酸残基位置，磷酸化将提供与含有SH2结构域的蛋白结合的位置(3).CDCP1是一个潜在的造血干细胞标记(4,5)。

1. Scherl-Mostageer, M. et al. (2001) Oncogene 20, 4402-8.
2. Ikeda, J.I. et al. (2006) J. Pathol. 210, 75-84.
3. Hooper, J.D. et al. (2003) Oncogene 22, 1783-94.
4. Conze, T. et al. (2003) Ann. N.Y. Acad. Sci. 996, 222-226.
5. Bühring, H.J. et al. (2004) Stem Cells 22, 334-343.

原厂资料：

Specificity / Sensitivity

Phospho-CDCP1 (Tyr707) Antibody recognizes endogenous levels of CDCP1 protein only when phosphorylated at Tyr707. This antibody does not cross-react with CDCP1 phosphorylated at Tyr734, Tyr743, or Tyr806, but may cross react with other phospho-tyrosine containing proteins. The identity of the 80 kDa band in the Cub1 antibody treated ZR-75-1 cell extract is not known.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Tyr707 of human CDCP1 protein. Antibodies are purified by protein A and peptide affinity chromatography

Background

CUB domain containing protein 1 (CDCP1, SIMA135) is a putative stem cell marker shown in research studies to be highly expressed in some human cancer cells and in both typical and atypical (cancerous) colons (1). Expression of CDCP1 may be epigenetically regulated, as methylation of promoter CpG sequences results in decreased CDCP1 expression (2). The corresponding CDCP1 gene encodes a glycoprotein that acts as a complex, multidomain transmembrane antigen. CDCP1 has three extracellular CUB domains that may be involved in cell adhesion or extracellular matrix interactions (1,3). Src-family kinases may phosphorylate CDCP1 at five tyrosine residues within its cytoplasmic domain to provide a potential binding site for SH2 domain-containing proteins (3). CDCP1 is a putative hematopoietic stem cell marker (4,5).

1. Scherl-Mostageer, M. et al. (2001) Oncogene 20, 4402-8.
2. Ikeda, J.I. et al. (2006) J. Pathol. 210, 75-84.
3. Hooper, J.D. et al. (2003) Oncogene 22, 1783-94.
4. Conze, T. et al. (2003) Ann. N.Y. Acad. Sci. 996, 222-226.
5. Bühring, H.J. et al. (2004) Stem Cells 22, 334-343.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM
NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C.
Do not aliquot the antibody