CTR1/SLC31A1 Antibody

• 产品编号：CST-13086S      品牌：CST       原厂货号：13086S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

CTR1/SLC31A1 Antibody可以识别内源性CTR1 (SLC31A1)总蛋白水平。多抗由合成肽段免疫动物产生，该肽段与人CTR1 (SLC31A1)蛋白Pro107邻近氨基酸序列一致。抗体由蛋白A和肽段亲和层析技术纯化得到。高亲和力铜摄取蛋白1(CTR1,SLC31A1)主要在小肠中通过介导饮食摄入铜以保持铜的动态平衡(1)。不同物种的CTR1都保守的含有一系列蛋氨酸富含重复序列和其他的氨基酸残基序列，并被认为对于铜的运输非常重要(2,3)。在哺乳动物细胞中，CTR1位于细胞膜和细胞囊泡内(3)。通过细胞膜将铜摄入细胞，CTR1被clathrin依赖的内吞作用所下调，且CTR1蛋白被降解(4)。研究提示，CTR1铜转运蛋白还介导了酵母和哺乳动物抗癌药物cisplatin的摄入，CTR1的下调会导致cisplatin的耐药性(5,6)。使用cisplatin治疗癌细胞能显著降低CTR1表达，从而降低细胞内cisplatin积累并导致某些人类癌细胞的cisplatin耐药性(7-9)。

1. Lee, J. et al. (2001) Proc Natl Acad Sci U S A 98, 6842-7.
2. Kim, B.E. et al. (2008) Nat Chem Biol 4, 176-85.
3. Lee, J. et al. (2002) J Biol Chem 277, 4380-7.
4. Petris, M.J. et al. (2003) J Biol Chem 278, 9639-46.
5. Ishida, S. et al. (2002) Proc Natl Acad Sci U S A 99, 14298-302.
6. Kuo, M.T. et al. (2007) Cancer Metastasis Rev 26, 71-83.
7. Abada, P. and Howell, S.B. (2010) Met Based Drugs 2010, 317581.
8. Yu, L. et al. (2011) Nan Fang Yi Ke Da Xue Xue Bao 31, 801-4.
9. Kalayda, G.V. et al. (2012) J Inorg Biochem 116, 1-10.

原厂资料：

Specificity / Sensitivity

CTR1/SLC31A1 Antibody recognizes endogenous levels of total CTR1 (SLC31A1) protein.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro107 of human CTR1 (SLC31A1) protein. Antibodies are purified by protein A and peptide affinity chromatography.

Background

The high affinity copper uptake protein 1 (CTR1, SLC31A1) helps maintain copper homeostasis by mediating dietary copper intake chiefly in the small intestine (1). A series of methionine-rich repeats and other residues are conserved among CTR1 genes across taxa, and are thought to be important for copper transport (2,3). In mammalian cells, CTR1 is localized to the plasma membrane and intracellular vesicles (3). Upon copper uptake via plasma membrane into cells, CTR1 is down regulated by clathrin-dependent endocytosis and degradation of CTR1 protein (4). Research studies suggest that the CTR1 copper transporter also mediates uptake of the anticancer drug cisplatin in yeast and mammals and that decreased CTR1 can result in the development of cisplatin resistance (5,6). Treatment of cancer cells with cisplatin can result in reduced CTR1 expression, which reduces cisplatin accumulation within cells and leads to cisplatin resistance in some human cancer cells (7-9).

1. Lee, J. et al. (2001) Proc Natl Acad Sci U S A 98, 6842-7.
2. Kim, B.E. et al. (2008) Nat Chem Biol 4, 176-85.
3. Lee, J. et al. (2002) J Biol Chem 277, 4380-7.
4. Petris, M.J. et al. (2003) J Biol Chem 278, 9639-46.
5. Ishida, S. et al. (2002) Proc Natl Acad Sci U S A 99, 14298-302.
6. Kuo, M.T. et al. (2007) Cancer Metastasis Rev 26, 71-83.
7. Abada, P. and Howell, S.B. (2010) Met Based Drugs 2010, 317581.
8. Yu, L. et al. (2011) Nan Fang Yi Ke Da Xue Xue Bao 31, 801-4.
9. Kalayda, G.V. et al. (2012) J Inorg Biochem 116, 1-10.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM
NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C.
Do not aliquot the antibody.