Drosophila ICE (drICE) Antibody

• 产品编号：CST-13085S      品牌：CST       原厂货号：13085S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

Drosophila ICE (drICE) Antibody 能够检测内源的 drICE 总蛋白，包括全长和裂解后的蛋白产物。该多抗采用合成的Drosophila ICE (drICE)蛋白Asp230 周围残基相对应的肽段免疫动物产生，该肽段与对应。抗体由蛋白A和肽段亲和层析技术纯化得到。黑腹果蝇的细胞死亡和哺乳动物细胞死亡一样，是受许多相同的刺激调控的（1）。果蝇的基因组包含七个caspase基因，三个编码半胱天冬酶，四个编码效应胱天蛋白酶（2）。果蝇的效应器caspase，即死亡caspase-1（DCP-1），是一个关键的细胞凋亡执行者。它参与了许多关键蛋白的蛋白水解的剪切，如多聚核酶（ADP-核糖）聚合酶（PARP）。 DCP-1的激活需要其非活动状态酶原蛋白水解成活性p22和p13片段（3）。研究比较体内drICE和DCP-1的活性表明，drICE相对于Dcp-1是一种更有效的细胞凋亡诱导剂，后者在确定细胞死亡率方面起到了重要作用（4）。

1. Steller, H. et al. (1994) Philos Trans R Soc Lond B Biol Sci 345, 247-50.
2. Hay, B.A. and Guo, M. (2006) Annu Rev Cell Dev Biol 22, 623-50.
3. Fraser, A.G. and Evan, G.I. (1997) EMBO J 16, 2805-13.
4. Florentin, A. and Arama, E. (2012) J Cell Biol 196, 513-27.

原厂资料：

Specificity / Sensitivity

Drosophila ICE (drICE) Antibody recognizes endogenous levels of total drICE protein, including both full-length and cleaved protein products.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Asp230 of Drosophila ICE (drICE) protein. Antibodies are purified by protein A and peptide affinity chromatography.

Background

Cell death in the fruit fly Drosophila melanogaster is regulated by many of the same stimuli as mammalian cell death (1). The Drosophila genome contains seven caspase genes; three encode initiator caspases and four encode effector caspases (reviewed in 2). drICE is a cysteine protease that cleaves baculovirus p35 and lamin DmO in vitro and acts downstream of rpr (3). drICE is proteolytically processed during apoptosis into active p21 and p12 subunits. Comparison of the in vivo activity between drICE and Dcp-1 has shown that drICE is a more effective inducer of apoptosis than Dcp-1, which plays a role in determining the rate of cell death (4).

1. Steller, H. et al. (1994) Philos Trans R Soc Lond B Biol Sci 345, 247-50.
2. Hay, B.A. and Guo, M. (2006) Annu Rev Cell Dev Biol 22, 623-50.
3. Fraser, A.G. and Evan, G.I. (1997) EMBO J 16, 2805-13.
4. Florentin, A. and Arama, E. (2012) J Cell Biol 196, 513-27.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM
NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C.
Do not aliquot the antibody