AGR2 (D9V2F) XP? Rabbit mAb

• 产品编号：CST-13062S      品牌：CST       原厂货号：13062S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

AGR2 (D9V2F) XP^® Rabbit mAb可以识别内源性的总AGR2蛋白。此单克隆抗体由合成肽段免疫动物产生，该肽段与人AGR2蛋白的Ala29邻近氨基酸残基序列一致.Anterior gradient homolog 2 (AGR2)是蛋白质二硫键异构酶（PDI）家族成员，是非洲爪蟾cement gland蛋白的同源物（1）。在正常人组织中，AGR2主要表达在小肠细胞中。它也在一些恶性腺瘤中过表达，包括乳腺癌、胰腺癌、卵巢癌、前列腺癌和食管癌（2-4）。体外和体内试验表明AGR2正向调控细胞生长和分裂，过表达AGR2可以促进细胞的转化（5,6）。AGR2还涉及了YAP1介导的双调蛋白表达上调，提示AGR2在EGF和Hippo 激酶通路中有发挥作用（6）。

1. Maresh, E.L. et al. (2010) BMC Cancer 10, 680.
2. Pizzi, M. et al. (2012) Appl Immunohistochem Mol Morphol 20, 31-6.
3. Wang, Z. et al. (2008) Cancer Res 68, 492-7.
4. Park, K. et al. (2011) Exp Mol Med 43, 91-100.
5. Vanderlaag, K.E. et al. (2010) Breast Cancer Res 12, R32.
6. Dong, A. et al. (2011) J Biol Chem 286, 18301-10.

原厂资料：

Homology

Species predicted to react based on 100% sequence homology: Monkey, Xenopus

Specificity / Sensitivity

AGR2 (D9V2F) XP^® Rabbit mAb recognizes endogenous levels of total AGR2 protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ala29 of human AGR2 protein.

Background

Anterior gradient homolog 2 (AGR2) is a member of the protein disulfide isomerase (PDI) family of proteins and a homolog of the Xenopus laevis cement gland protein (1). In normal human tissues, AGR2 is expressed most abundantly in intestinal cells. Research studies have found AGR2 is overexpressed in a number of adenocarcinomas, including those derived from breast, pancreas, ovary, prostate and esophagus (2-4). In vitro and in vivo studies have shown that AGR2 positively regulates cell growth and division, while its overexpression can promote cell transformation (5,6). The latter functions of AGR2 were shown to involve YAP1-mediated up-regulation of amphiregulin expression, implicating AGR2 in both the EGF and Hippo kinase signaling pathways (6).

1. Maresh, E.L. et al. (2010) BMC Cancer 10, 680.
2. Pizzi, M. et al. (2012) Appl Immunohistochem Mol Morphol 20, 31-6.
3. Wang, Z. et al. (2008) Cancer Res 68, 492-7.
4. Park, K. et al. (2011) Exp Mol Med 43, 91-100.
5. Vanderlaag, K.E. et al. (2010) Breast Cancer Res 12, R32.
6. Dong, A. et al. (2011) J Biol Chem 286, 18301-10.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150
mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02%
sodium azide. Store at –20°C. Do not aliquot the antibody