DAZL (D2A4) Rabbit mAb (IHC Specific)

• 产品编号：CST-13057S      品牌：CST       原厂货号：13057S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

DAZL (D2A4) (IHC Specific) Rabbit mAb特异性识别内源性的总DAZL蛋白。单克隆抗体由合成肽段免疫动物产生，该肽段与人DAZL蛋白的羧基端邻近残基序列一致。人DAZ (Deleted in Azoospermia)基因家族含有至少三个成员编码含有一个共同的RNA识别基序的RNA结合蛋白(1)。DAZ的常染色体同源蛋白，DAZL (DAZ-like)在生殖细胞中特异性表达，并对胚胎发育时和两性成体的生殖细胞成熟期的生殖细胞系发育限定至关重要(2,3)。DAZL可能会直接募集poly(A)结合蛋白(PABPs)以在生殖细胞发育时激活沉默mRNAs(2)。 人类不孕不育症中最常见的分子病因就是Y染色体上的DAZ 基因缺失(4,5).

1. Yen, P.H. (2004) Int J Androl 27, 125-9.
2. Collier, B. et al. (2005) EMBO J 24, 2656-66.
3. Brook, M. et al. (2009) Reproduction 137, 595-617.
4. Reijo, R. et al. (1995) Nat Genet 10, 383-93.
5. Cooke, H.J. and Elliott, D.J. (1997) Trends Genet 13, 87-9.

原厂资料：

Homology

Species predicted to react based on 100% sequence homology: Rat

Specificity / Sensitivity

DAZL (D2A4) (IHC Specific) Rabbit mAb recognizes endogenous levels of total DAZL protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human protein.

Background

The human DAZ (Deleted in Azoospermia) gene family contains at least three members that encode RNA-binding proteins with a common RNA-recognition motif (1). An autosomal homolog of DAZ, DAZL (DAZ-like), is specifically expressed in germ cells and is essential for the specification of the germ cell lineage during embryogenesis and during gametogenesis in adults of both sexes (2,3). DAZL may function by directly recruiting poly(A)-binding proteins (PABPs) in order to activate silent mRNAs during germ cell development (2). Deletions encompassing the Y chromosomal DAZgenes are the most common molecularly defined cause of infertility in humans (4,5).

1. Yen, P.H. (2004) Int J Androl 27, 125-9.
2. Collier, B. et al. (2005) EMBO J 24, 2656-66.
3. Brook, M. et al. (2009) Reproduction 137, 595-617.
4. Reijo, R. et al. (1995) Nat Genet 10, 383-93.
5. Cooke, H.J. and Elliott, D.J. (1997) Trends Genet 13, 87-9.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150
mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02%
sodium azide. Store at –20°C. Do not aliquot the antibody.