Phospho-ATM (Ser1981) (D25E5) Rabbit mAb

• 产品编号：CST-13050S      品牌：CST       原厂货号：13050S
• 产品分类：抗体 > 一抗 > 磷酸化抗体
• 应用分类：

描述：

Phospho-ATM (Ser1981) (D25E5) Rabbit mAb可以识别丝氨酸1981 被磷酸化的内源性ATM蛋白。单克隆抗体由合成肽段免疫动物产生，该肽段与人ATM蛋白的丝氨酸1981邻近残基一致。共济失调毛细血管扩张突变激酶（ATM）是一种丝氨酸/苏氨酸激酶可以调控细胞周期检验点和DNA修复（1）。DNA双链缺口会诱导ATM蛋白丝氨酸1981自磷酸化从而激活ATM。ATM激酶调控一组涉及细胞周期检验点调控，凋亡和DNA修复的蛋白。已知的底物包括p53，Chk2，Chk1，CtIP，4E-BP1，BRCA1，RPA3，H2A.X，SMC1，FANCD2，Rad17，Artemis,Nbs1,和PP1的I-2调控亚基(1,2).ATM基因突变会导致共济失调毛细管扩张（AT），一种常染色体隐形遗传病表现为肌肉运动和神经退行性变的不协调。AT病人细胞表现出DNA损伤诱导的细胞周期检验点激活，对辐射敏感，以及易发生染色质断裂(3,4).

1. Lee, J.H. and Paull, T.T. (2007) Oncogene 26, 7741-8.
2. Tang, X. et al. (2008) Mol Cell Biol 28, 2559-66.
3. Shiloh, Y. (1997) Annu Rev Genet 31, 635-62.
4. Petrini, J.H. (2000) Curr Opin Cell Biol 12, 293-6.

原厂资料：

Homology

Species predicted to react based on 100% sequence homology: Monkey

Specificity / Sensitivity

Phospho-ATM (Ser1981) (D25E5) Rabbit mAb recognizes endogenous levels of ATM protein only when phosphorylated at Ser1981.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser1981 of human ATM protein.

Background

Ataxia telangiectasia mutated kinase (ATM) is a serine/threonine kinase that regulates cell cycle checkpoints and DNA repair (1). Activation of ATM by autophosphorylation on Ser1981 occurs in response to exposed DNA double stranded breaks. ATM kinase regulates a number of proteins involved in cell cycle checkpoint control, apoptosis, and DNA repair. Known substrates include p53, Chk2, Chk1, CtIP, 4E-BP1, BRCA1, RPA3, H2A.X, SMC1, FANCD2, Rad17, Artemis, Nbs1, and the I-2 regulatory subunit of PP1 (1,2). Mutations in the corresponding ATM gene result in ataxia telangiectasia (AT), an autosomal recessive disease characterized by uncoordinated muscle movement and neurodegeneration. Cells from AT patients display defective DNA damage-induced checkpoint activation, sensitivity to radiation, and a higher frequency of chromosome breakage (3,4).

1. Lee, J.H. and Paull, T.T. (2007) Oncogene 26, 7741-8.
2. Tang, X. et al. (2008) Mol Cell Biol 28, 2559-66.
3. Shiloh, Y. (1997) Annu Rev Genet 31, 635-62.
4. Petrini, J.H. (2000) Curr Opin Cell Biol 12, 293-6.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150
mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02%
sodium azide. Store at –20°C. Do not aliquot the antibody