Rab25 (D4P6P) XP? Rabbit mAb

• 产品编号：CST-13048S      品牌：CST       原厂货号：13048S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

Rab25 (D4P6P) XP^® Rabbit mAb可以识别内源性总的Radb25蛋白。单克隆抗体由合成肽段免疫动物产生，该肽段与人Rab25蛋白羧基端附近氨基酸一致。Rab11a, Rab11b, 和Rab25是小Ras-like GTPases 的Rab11超家族成员。不像广泛表达的Rab11，经典的Rab25表达似乎限制在胃肠粘膜，肾和肺组织（1）。Rab25可以和顶端回收囊泡联合帮助调控顶端囊泡示踪（2,3）。研究表明非典型Rab25的表达与多种癌症相关。Rab25表达增加和卵巢癌，乳腺癌中的侵袭性增长相关，Rab25可能会抑制细胞凋亡并促进癌细胞增殖以及通过调控囊泡运输和细胞活性来增进癌细胞侵润（4-7）。Rab25和β1整合素蛋白结合能促进囊泡介导的转运，这个转运过程将整合素转移到伪足端细胞膜，维持肿瘤细胞侵润端的功能（8）。相反地，已经报道的多种乳腺癌细胞中Rab25的表达缺失对于癌症的发病机理尚不明确（9）。

1. Goldenring, J.R. et al. (1993) J Biol Chem 268, 18419-22.
2. Casanova, J.E. et al. (1999) Mol Biol Cell 10, 47-61.
3. Wang, X. et al. (2000) J Biol Chem 275, 29138-46.
4. Cheng, K.W. et al. (2004) Nat Med 10, 1251-6.
5. Cheng, K.W. et al. (2005) Cancer Res 65, 2516-9.
6. Chia, W.J. and Tang, B.L. (2009) Biochim Biophys Acta 1795, 110-6.
7. Tang, B.L. and Ng, E.L. (2009) Cell Motil Cytoskeleton 66, 365-70.
8. Caswell, P.T. et al. (2007) Dev Cell 13, 496-510.
9. Cheng, J.M. et al. (2010) Int J Cancer 126, 2799-812.

原厂资料：

Specificity / Sensitivity

Rab25 (D4P6P) XP^® Rabbit mAb recognizes endogenous levels of total Rab25 protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human Rab25 protein.

Background

Rab11a, Rab11b, and Rab25 are members of the Rab11 subfamily of small Ras-like GTPases. Unlike universally expressed Rab11, typical Rab25 expression appears to be limited to gastrointestinal mucosa, kidney, and lung (1). Rab25 can associate with apical recycling vesicles to help regulate apical vesicle trafficking (2,3). Research studies indicate that atypical Rab25 expression can be associated with various forms of cancer. Increased Rab25 expression is associated with aggressive growth in ovarian and breast cancer, where Rab25 may inhibit apoptosis and promote cancer cell proliferation and invasion through regulation of vesicle transport and cellular motility (4-7). Interaction between Rab25 and β1 integrin promotes vesicle-mediated transport of integrin to pseudopodial tip membranes, fostering the persistent invasion of tumor cells (8). Conversely, the reported loss of Rab25 expression in a number of breast cancer cases has an unclear effect on cancer pathogenesis (9).

1. Goldenring, J.R. et al. (1993) J Biol Chem 268, 18419-22.
2. Casanova, J.E. et al. (1999) Mol Biol Cell 10, 47-61.
3. Wang, X. et al. (2000) J Biol Chem 275, 29138-46.
4. Cheng, K.W. et al. (2004) Nat Med 10, 1251-6.
5. Cheng, K.W. et al. (2005) Cancer Res 65, 2516-9.
6. Chia, W.J. and Tang, B.L. (2009) Biochim Biophys Acta 1795, 110-6.
7. Tang, B.L. and Ng, E.L. (2009) Cell Motil Cytoskeleton 66, 365-70.
8. Caswell, P.T. et al. (2007) Dev Cell 13, 496-510.
9. Cheng, J.M. et al. (2010) Int J Cancer 126, 2799-812.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150
mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02%
sodium azide. Store at –20°C. Do not aliquot the antibody