AKR1C2 Antibody

• 产品编号：CST-13035S      品牌：CST       原厂货号：13035S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

AKR1C2抗体可以识别内源性AKR1C2总蛋白水平。多抗由合成肽段免疫动物产生，该肽段与人AKR1C2蛋白Val61附近氨基酸一致。抗体由蛋白A和肽段亲和层析技术纯化得到。醛-酮还原酶家族1成员C2(AKR1C2)是醛-酮还原酶超家族的一员，以NADPH依赖的方式代谢一系列的底物。AKR1C2是3-α-羟类固醇脱氢酶（3α-HSD）负责代谢和失活雄激素5α-dihydrotestosterone (DHT)以形成3α-androstanediol(1)。研究表明在某些晚期前列腺癌病例中AKR1C2表达上升(2)。AKR1C2和相关的还原酶，AKR1C3，涉及了在前列腺癌中通过降低前列腺素D2变为更稳定的9α, 11β-PGF2α而增强PI3K/Akt信号(2)。此外，研究证明在某些卵巢子宫内膜异位症标本中AKR1C2表达增加，提示AKR1C2在卵巢子宫内膜异位症中促进了孕激素的代谢(3)。

1. Rizner, T.L. et al. (2003) Chem Biol Interact 143-144, 401-9.
2. Wang, S. et al. (2008) Mol Cell Endocrinol 289, 60-6.
3. Hevir, N. et al. (2011) Chem Biol Interact 191, 217-26.

原厂资料：

Specificity / Sensitivity

AKR1C2 Antibody recognizes endogenous levels of total AKR1C2 protein.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Val61 of human AKR1C2 protein. Antibodies are purified by protein A and peptide affinity chromatography.

Background

Aldo-keto reductase family 1 member C2 (AKR1C2) is a member of a large superfamily of aldo-keto reductases that metabolize an array of substrates in a NADPH-dependent manner. AKR1C2 is the 3-α-hydroxysteroid dehydrogenase (3α-HSD) responsible for metabolizing and inactivating the androgen hormone 5α-dihydrotestosterone (DHT) to 3α-androstanediol (1). Research studies demonstrate an increase in AKR1C2 expression in cases of advanced prostate cancer (2). Both AKR1C2 and the related reductase, AKR1C3, are implicated in enhancing PI3K/Akt signaling in prostate cancer by reducing prostaglandin D2 to the more stable 9α, 11β-PGF2α (2). In addition, research studies demonstrate an increase in AKR1C2 expression in some ovarian endometriosis specimens, suggesting the participation of AKR1C2 in the promotion of progesterone metabolism in ovarian endometriosis (3).

1. Rizner, T.L. et al. (2003) Chem Biol Interact 143-144, 401-9.
2. Wang, S. et al. (2008) Mol Cell Endocrinol 289, 60-6.
3. Hevir, N. et al. (2011) Chem Biol Interact 191, 217-26.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM
NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C.
Do not aliquot the antibody.