FoxC2 (D4D4) Rabbit mAb

• 产品编号：CST-12974S      品牌：CST       原厂货号：12974S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

FoxC2 (D4D4) Rabbit mA兔单抗可以识别内源性FoxC2总蛋白水平。合成对应人FoxC2蛋白的Leu278及其邻近氨基酸序列的肽段免疫动物获得单抗。Forkhead box(Fox)蛋白是一类含有一个被称为Forkhead的翼状螺旋DNA结合结构域，在进化上保守的转录因子家族蛋白(1)。人体中，有超过40种已知的Fox蛋白家族成员，分为19个亚家族，在多样化和高度专业化的生物环境中参与调节了整个发育过程中的基因转录(2)。突变破坏Fox基因家族会随之产生多种人类疾病，包括免疫功能障碍，不育，言语/语言障碍和癌症(3,4)。脂肪生成过程会调控FoxC2和一些别的Fox转录因子的表达，提示它们参与了脂肪细胞分化(5)。白色脂肪组织(WAT)和棕色脂肪组织(BAT)过表达FoxC2的转基因小鼠模型表现出腹腔WAT减少的特点(6)。BAT特异基因Ucp1在这些转基因小鼠的腹腔脂肪中表达上调，导致它们具有了BAT的组化特征。这些证据提示FoxC2能发挥一种抗肥胖和饮食诱导的胰岛素抵抗的保护机制作用(6)。

1. Myatt, S.S. and Lam, E.W. (2007) Nat Rev Cancer 7, 847-59.
2. Jackson, B.C. et al. (2010) Hum Genomics 4, 345-52.
3. Hannenhalli, S. and Kaestner, K.H. (2009) Nat Rev Genet 10, 233-40.
4. Benayoun, B.A. et al. (2011) Trends Genet 10, 224-32.
5. Gerin, I. et al. (2009) J Biol Chem 284, 10755-63.
6. Cederberg, A. et al. (2001) Cell 106, 563-73.

原厂资料：

Specificity / Sensitivity

FoxC2 (D4D4) Rabbit mAb recognizes endogenous levels of total FoxC2 protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Leu278 of human FoxC2 protein.

Background

Forkhead box (Fox) proteins are a family of evolutionarily conserved transcription factors defined by the presence of a winged helix DNA binding domain called a Forkhead box (1). In humans, there are over 40 known Fox protein family members, divided into 19 subfamilies, which have evolved to regulate gene transcription in diverse and highly specialized biological contexts throughout development (2). Mutations that disrupt the expression of Fox gene family members have consequently been implicated in a broad array of human disorders, including immunological dysfunction, infertility, speech/language disorders, and cancer (3,4).The expression of FoxC2 and several other Fox transcription factors are regulated during adipogenesis, suggesting their involvement in adipocyte differentiation (5). A transgenic mouse model exhibiting over expression of FoxC2 in white adipose tissue (WAT) and brown adipose tissue (BAT) showed a reduction of the intra-abdominal WAT depot (6). The BAT-specific gene Ucp1 is upregulated in the intra-abdominal WAT depot of these transgenic mice, resulting in histological features similar to those seen in BAT. This evidence suggests that FoxC2 can play a protective role against obesity and diet-induced insulin resistance (6).

1. Myatt, S.S. and Lam, E.W. (2007) Nat Rev Cancer 7, 847-59.
2. Jackson, B.C. et al. (2010) Hum Genomics 4, 345-52.
3. Hannenhalli, S. and Kaestner, K.H. (2009) Nat Rev Genet 10, 233-40.
4. Benayoun, B.A. et al. (2011) Trends Genet 10, 224-32.
5. Gerin, I. et al. (2009) J Biol Chem 284, 10755-63.
6. Cederberg, A. et al. (2001) Cell 106, 563-73.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150
mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02%
sodium azide. Store at –20°C. Do not aliquot the antibody.