Phospho-Syk (Tyr525/526) (C87C1) Rabbit mAb (Pacific Blue? Conjugate)

• 产品编号：CST-12967S      品牌：CST       原厂货号：12967S
• 产品分类：抗体 > 一抗 > 染料标记抗体
• 应用分类：

描述：

Homology

Species predicted to react based on 100% sequence homology: Mouse, Rat

Specificity / Sensitivity

Phospho-Syk (Tyr525/526) (C87C1) Rabbit mAb (Pacific Blue™ Conjugate) detects endogenous levels of Syk protein only when phosphorylated at Tyr525/526 of human Syk or Tyr519/520 of mouse Syk. It also detects Syk protein when singly phosphorylated at Tyr526 of human Syk or Tyr520 of mouse Syk. It does not cross-react with other tyrosine-phosphorylated protein tyrosine kinases.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Tyr525/526 of human Syk protein.

Description

This Cell Signaling Technology antibody is conjugated to Pacific Blue™ fluorescent dye and tested in-house for direct flow cytometry in human cells. The antibody is expected to exhibit the same species cross-reactivity as the unconjugated antibody Phospho-Syk (Tyr525/526) (C87C1) Rabbit mAb #2710.

原厂资料：

Homology

Species predicted to react based on 100% sequence homology: Mouse, Rat

Specificity / Sensitivity

Phospho-Syk (Tyr525/526) (C87C1) Rabbit mAb (Pacific Blue™ Conjugate) detects endogenous levels of Syk protein only when phosphorylated at Tyr525/526 of human Syk or Tyr519/520 of mouse Syk. It also detects Syk protein when singly phosphorylated at Tyr526 of human Syk or Tyr520 of mouse Syk. It does not cross-react with other tyrosine-phosphorylated protein tyrosine kinases.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Tyr525/526 of human Syk protein.

Description

This Cell Signaling Technology antibody is conjugated to Pacific Blue™ fluorescent dye and tested in-house for direct flow cytometry in human cells. The antibody is expected to exhibit the same species cross-reactivity as the unconjugated antibody Phospho-Syk (Tyr525/526) (C87C1) Rabbit mAb #2710.

Background

Syk is a protein tyrosine kinase that plays an important role in intracellular signal transduction in hematopoietic cells (1-3). Syk interacts with immunoreceptor tyrosine-based activation motifs (ITAMs) located in the cytoplasmic domains of immune receptors (4). It couples the activated immunoreceptors to downstream signaling events that mediate diverse cellular responses, including proliferation, differentiation and phagocytosis (4). There is also evidence of a role for Syk in nonimmune cells and investigators have indicated that Syk is a potential tumor suppressor in human breast carcinomas (5). Tyr323 is a negative regulatory phosphorylation site within the SH2-kinase linker region in Syk. Phosphorylation at Tyr323 provides a direct binding site for the TKB domain of Cbl (6,7). Tyr352 of Syk is involved in the association of PLCγ1 (8). Tyr525 and Tyr526 are located in the activation loop of the Syk kinase domain; phosphorylation at Tyr525/526 of human Syk (equivalent to Tyr519/520 of mouse Syk) is essential for Syk function (9).

1. Cheng, A.M. and Chan, A.C. (1997) Curr Opin Immunol 9, 528-33.
2. Kurosaki, T. (1997) Curr Opin Immunol 9, 309-18.
3. Chu, D.H. et al. (1998) Immunol Rev 165, 167-80.
4. Turner, M. et al. (2000) Immunol Today 21, 148-54.
5. Coopman, P.J. et al. (2000) Nature 406, 742-7.
6. Deckert, M. et al. (1998) J Biol Chem 273, 8867-74.
7. Rao, N. et al. (2001) EMBO J 20, 7085-95.
8. Law, C.L. et al. (1996) Mol Cell Biol 16, 1305-15.
9. Zhang, J. et al. (2000) J Biol Chem 275, 35442-7.

注意事项：

Storage: Supplied in PBS (pH 7.2), less than 0.1% sodium
azide and 2 mg/ml BSA. Store at 4°C. Do not aliquot the anti-
body. Protect from light. Do not freeze.