PCK1 (D12F5) Rabbit mAb

• 产品编号：CST-12940S      品牌：CST       原厂货号：12940S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

PCK1 (D12F5) Rabbit mAb可以识别内源性PCK1总蛋白水平。单克隆抗体由合成肽段免疫动物产生，该肽段与人PCK1蛋白羧基端附近氨基酸一致。磷酸烯醇式丙酮酸羧激酶1 (PCK1, PEPCK1或PEPCK-C)是一种细胞质酶负责草酰乙酸转化为磷酸烯醇式丙酮酸(1)。PCK1参与调控肝脏的糖异生作用的限速步骤，能从非碳水化合物底物如乳酸和甘油产生葡萄糖(2).去乙酰基SirT1刺激PCK1和葡萄糖-6-磷酸酶转录以激活糖质新生(3)。根据营养状况，STAT3可以抑制PCK1和葡萄糖-6-磷酸酶的表达，抑制糖异生(4)。相对高浓度的葡萄糖浓度会导致PCK1被P300乙酰转移酶乙酰化，促进PCK1和E3连接酶UBR5的相互作用导致PCK1的不稳定性(5)。

1. Caton, P.W. et al. (2009) Life Sci 84, 738-44.
2. Yoon, J.C. et al. (2001) Nature 413, 131-8.
3. Rodgers, J.T. et al. (2005) Nature 434, 113-8.
4. Nie, Y. et al. (2009) Nat Cell Biol 11, 492-500.
5. Jiang, W. et al. (2011) Mol Cell 43, 33-44.

原厂资料：

Specificity / Sensitivity

PCK1 (D12F5) Rabbit mAb recognizes endogenous levels of total PCK1 protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human PCK1 protein.

Background

Phosphoenolpyruvate carboxykinase 1 (PCK1, PEPCK1 or PEPCK-C) is a cytosolic enzyme responsible for the conversion of oxaloacetate to phosphoenolpyruvate (1). PCK1 is involved in controlling the rate-limiting step of gluconeogenesis in the liver, which generates glucose from non-carbohydrate substrates such as lactate and glycerol (2). The deacetylase SirT1 stimulates transcription of PCK1 and glucose-6-phosphatase to activate gluconeogenesis (3). Depending on nutritional state, Stat3 can inhibit PCK1 and glucose-6-phosphatase expression and suppress gluconeogenesis (4). Relatively high glucose concentration can result in acetylation of PCK1 by P300 acetyltransferase, promoting an interaction between PCK1 and the E3 ligase UBR5 that leads to the PCK1 destabilization (5).

1. Caton, P.W. et al. (2009) Life Sci 84, 738-44.
2. Yoon, J.C. et al. (2001) Nature 413, 131-8.
3. Rodgers, J.T. et al. (2005) Nature 434, 113-8.
4. Nie, Y. et al. (2009) Nat Cell Biol 11, 492-500.
5. Jiang, W. et al. (2011) Mol Cell 43, 33-44.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150
mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02%
sodium azide. Store at –20°C. Do not aliquot the antibody.