O-GlcNAc (CTD110.6) Mouse mAb?(HRP Conjugate)

• 产品编号：CST-12938S      品牌：CST       原厂货号：12938S
• 产品分类：抗体 > 一抗 > 酶标记一抗 > 辣根过氧化物酶（HRP）
• 应用分类：

描述：

O-GlcNAc (CTD110.6) Mouse mAb 鼠单抗(HRP Conjugate)能够特异性识别内源性蛋白上以β-O-glycosidic 与丝氨酸和苏氨酸结合O-GlcNAc 。单克隆抗体由合成肽段免疫动物产生，该肽段含有serine-O-linked N-acetylglucosamine (O-GlcNAc)。Cell Signaling Technology公司抗体通过胺基基团和辣根过氧化物酶(HRP)的碳水化合物组偶联。HRP偶联抗体预估和没有偶联的O-GlcNAc (CTD110.6) Mouse mAb #9875有相同的交叉反应性。O-GlcNAcylation是一种翻译后修饰，其中N-乙酰基-D-葡糖胺(GlcNAc)与细胞质和细胞核蛋白的丝氨酸或苏氨酸残基通过共价键连接(1,2)。这种修饰在多种细胞过程中都非常重要，包括代谢，细胞生长和形态发育，凋亡和转录(2,3)并且在癌症中发挥了关键作用(1)。

1. Comer, F.I. et al. (2001) Anal Biochem 293, 169-77.
2. Slawson, C. and Hart, G.W. (2011) Nat Rev Cancer 11, 678-84.
3. Capotosti, F. et al. (2011) Cell 144, 376-88.

原厂资料：

Specificity / Sensitivity

O-GlcNAc (CTD110.6) Mouse mAb (HRP Conjugate) specifically recognizes endogenous levels of O-GlcNAc on proteins in β-O-glycosidic linkage to both serine and threonine.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a peptide containing serine-O-linked N-acetylglucosamine (O-GlcNAc).

Description

This Cell Signaling Technology^® antibody is conjugated to the carbohydrate groups of horseradish peroxidase (HRP) via its amine groups. The HRP conjugated antibody is expected to exhibit the same species cross-reactivity as the unconjugated O-GlcNAc (CTD110.6) Mouse mAb #9875.

Background

O-GlcNAcylation is a post-translational modification where N-acetyl-D-glucosamine (GlcNAc) is covalently linked to cytoplasmic and nuclear proteins at serine or threonine residues (1,2). This modification is important in many cellular processes including metabolism, cell growth and morphogenesis, apoptosis and transcription (2,3) and has been implicated to play a critical role in cancer (1).

1. Comer, F.I. et al. (2001) Anal Biochem 293, 169-77.
2. Slawson, C. and Hart, G.W. (2011) Nat Rev Cancer 11, 678-84.
3. Capotosti, F. et al. (2011) Cell 144, 376-88.

注意事项：

Storage: Supplied in 136 mM NaCl, 2.6 mM KCl, 12 mM
sodium phosphate (pH 7.4) dibasic, 2 mg/ml BSA, and 50%
glycerol. Store at –20°C. Do not aliquot the antibodies