Neuronal Scaffold Proteins Antibody Sampler Kit

• 产品编号：CST-12882S      品牌：Cell Signaling Technology       原厂货号：12882S
• 产品分类：抗体 > 一抗 > 复合抗体试剂盒
• 应用分类：

其它组分：

描述：

PSD95 (D27E11) XP®Rabbit mAb兔单抗可以识别内源性的总PSD95蛋白。SHANK2 Antibody 兔多抗可以检测内源性的总SHANK2蛋白。Homer1 Antibody 兔多抗可以检测内源性的总Homer1蛋白，该抗体可能也可以检测到Homer1c亚型。Spinophilin Antibody 兔多抗可以检测内源性的总Spinophilin蛋白。多克隆抗体是由合成的人SHANK2蛋白Val537邻近肽段、人Homer1蛋白Glu130邻近肽段或人spinophilin蛋白Ala369邻近肽段免疫动物而制备的。抗体经过蛋白A和肽亲和层析纯化。单克隆抗体是由合成的人PSD95蛋白Gly99邻近邻近肽段免疫动物而制备的。Neuronal Scaffold Proteins Antibody Sampler Kit 小包装抗体试剂盒提供了一种经济的方式检测四种主要的支架蛋白。该试剂盒包含了足够的一抗以满足每款抗体用于4张膜的免疫印迹实验。支架蛋白由蛋白相互作用结构域组成，将信号通路的多个组分结合起来形成信号传导复合物。组织起来的信号分子可以加强信号特异性和传递速度。支架蛋白是神经突触的核心成分，突触蛋白的动态示踪在支架蛋白处发生。支架蛋白突变对于突触结构和功能有重要影响。Postsynaptic density protein 95 (PSD95)是一种膜相关鸟苷酸激酶（MAGUK）蛋白质家族的成员，PSD95是一种scaffolding蛋白，参与突触后密集复合物的装配和功能(1,2)。SHANK蛋白作为神经元post-synaptic density（PSD）的支架，在发育中兴奋性突触组装PSD时发挥了关键作用（3,4）。尽管招募SHANK蛋白到突触不依赖其与Homer的相互作用（5），SHANK1适当的突触靶向性是被其与PDZ结构域和PSD蛋白之间的相互作用所介导的（6）。Homer蛋白(1-3) 是支架蛋白，由EVH蛋白结合域、螺旋域和亮氨酸拉链结构域组成。EVH结构域是蛋白质结合的模块，可以结合富含脯氨酸的G蛋白偶联受体（GPCRs）、肌醇1,4,5-三磷酸（IP3）受体（IP3Rs）、ryanodine受体、TRP通道的PPXXF，PPXF，和LPSSP模体（7,8）。卷曲螺旋结构域和亮氨酸拉链结构由Homers和信号蛋白复合物多聚而成。Spinophilin是一个蛋白磷酸酶1调节蛋白，可以和大量的蛋白相互作用，包括离子通道组分和G蛋白偶联受体(GPCRs)。Spinophilin也可以和肌动蛋白丝相互作用；Spinophilin的Ser94和Ser177磷酸化会破坏其相互作用(9,10)。

原厂资料：

Specificity / Sensitivity

PSD95 (D27E11) XP^® Rabbit mAb detects endogenous levels of total PSD95 protein. SHANK2 Antibody recognizes endogenous levels of total SHANK2 protein. Homer1 Antibody detects endogenous levels of total Homer1 protein. This antibody may also detect isoform Homer1c. Spinophilin Antibody recognizes endogenous levels of total spinophilin protein.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Val537 of human SHANK2 protein, residues surrounding Glu130 of human Homer1 protein, or residues surrounding Ala369 of human spinophilin protein. Antibodies are purified by protein A and peptide affinity chromatography. Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly99 of human PSD95.

Description

The Neuronal Scaffold Proteins Antibody Sampler Kit provides an economical means of evaluating four major scaffolding proteins. The kit includes enough primary antibody to perform four western blot experiments.

Background

Scaffold proteins are composed of protein-interaction domains that tether multiple components of a signaling pathway to form signal transduction complexes. This organization of signaling molecules can help enhance signaling specificity and speed. Scaffold proteins are central components in neuronal synapses, where dynamic trafficking of synaptic proteins occurs. Mutations in scaffold proteins could have significant impact on synaptic structure and function. Postsynaptic density protein 95 (PSD95) is a member of the membrane-associated guanylate kinase (MAGUK) family of proteins and a scaffolding protein involved in the assembly and function of the postsynaptic density complex (1,2). SHANK proteins act as scaffolds at the neuronal post-synaptic density (PSD), where they play a critical role in PSD assembly of excitatory synapses during development (3,4). While recruitment of SHANK proteins to the synapse is independent of their interaction with Homer (5), proper synaptic targeting of SHANK1 is mediated by interactions between its PDZ domain and PSD proteins (6). Homer proteins (1-3) are scaffolds, composed of an EVH protein–binding domain, a coiled-coil domain and a leucine zipper domain. The EVH domain is a protein-protein binding module that binds to the proline-rich motifs of G-protein–coupled receptors (GPCRs), inositol 1,4,5-triphosphate (IP3) receptors (IP3Rs), ryanodine receptors, and TRP channels (7,8). The coiled-coil and the leucine zipper domains cause multimerization of Homers and assemble signaling proteins complexes. Spinophilin is a protein phosphatase 1 regulatory protein that interacts with a large number of proteins, including ion channel components and G-protein-coupled receptors (GPCRs). Spinophilin also interacts with actin filaments; phosphorylation of spinophilin at Ser94 and Ser177 disrupts this interaction (9,10).

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150
mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02%
sodium azide. Store at –20°C. Do not aliquot the antibodies.
Recommended Antibody Dilutions:
Western blotting  1:1000