MRP4/ABCC4 (D1Z3W) Rabbit mAb

• 产品编号：CST-12857S      品牌：CST       原厂货号：12857S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

ABCC4 (D1Z3W) Rabbit mAb兔单抗可以识别内源性ABCC4总蛋白水平。该抗体在某些细胞系中会形成47kDa的非特异性条带。单克隆抗体由合成肽段免疫动物产生，该肽段与人ABCC4a蛋白Gly192附近氨基酸序列一致。ABCC4是ATP结合盒（ABC）转运蛋白家族的成员。ABC可以利用ATP水解的能量跨细胞膜蛋白转运多种分子。ABC蛋白包括七个超家族：ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, 和White(1)。ABCC4属于MRP亚家族，参与多种药物抵抗，也被命名为MRP4。ABCC4广泛表达于包括前列腺、肾远端小管、星型胶质细胞、脑毛细血管内皮细胞、血小板和多种癌细胞系等细胞和组织中（2-4）。ABCC4接到多种内源性和异种生物阴离子的流出转运（5）。底物的多样性决定了ABCC4的生物功能。它能调节人白细胞的cAMP水平，因此可以控制白细胞的增殖和分化（6）。ABCC4也可以使得COX缺陷的胰腺癌细胞能获得外源性的前列腺素（7）。研究显示ABCC4在耐药的癌细胞中表达上调，这使得它成为一个治疗癌症的潜在位点（8,9）。ABCC4定位于细胞膜和胞内膜结构（10）。研究还发现ABCC4在Kawasaki病中的致病机制，Kawasaki病是一种以血管炎为特点的儿童疾病（11）。

1. Nakanishi, T. Cancer Genomics Proteomics 4, 241-54.
2. Kool, M. et al. (1997) Cancer Res 57, 3537-47.
3. Lee, K. et al. (1998) Cancer Res 58, 2741-7.
4. Nies, A.T. et al. (2004) Neuroscience 129, 349-60.
5. Giacomini, K.M. et al. (2010) Nat Rev Drug Discov 9, 215-36.
6. Copsel, S. et al. (2011) J Biol Chem 286, 6979-88.
7. Omura, N. et al. (2010) Mol Cancer Res 8, 821-32.
8. Bronger, H. et al. (2005) Cancer Res 65, 11419-28.
9. Hagmann, W. et al. (2009) Pancreatology 9, 136-44.
10. Rius, M. et al. (2008) J Pharmacol Exp Ther 324, 86-94.
11. Khor, C.C. et al. (2011) J Med Genet 48, 467-72.

原厂资料：

Specificity / Sensitivity

ABCC4 (D1Z3W) Rabbit mAb recognizes endogenous levels of total ABCC4 protein. This antibody cross-reacts with a nonspecific band at 47 kDa in some cell lines.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly192 of human ABCC4 protein.

Background

ABCC4 is a member of the ATP-binding Cassette (ABC) transporter family. ABC proteins transport various molecules across cellular membranes by utilizing the energy generated from ATP hydrolysis. There are seven subfamilies of ABC proteins: ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, and White (1). ABCC4 belongs to the MRP subfamily, which is involved in multi-drug resistance, hence it is also named MRP4. ABCC4 is widely expressed in tissues including prostate, kidney proximal tubules, astrocytes and capillary endothelial cells of the brain, platelets, and many cancer cell lines (2-4). ABCC4 mediates efflux transport of a wide variety of endogenous and xenobiotic organic anionic compounds (5). The diversity of substrates determines the biological functions of ABCC4. It regulates cAMP levels in human leukemia cells, thereby controlling the proliferation and differentiation of leukemia cells (6). ABCC4 also enables COX deficient pancreatic cancer cells to obtain exogenous prostaglandins (7). Research studies have shown that ABCC4 expression is elevated in drug resistant cancer cells, which makes it a potential target for cancer therapy (8,9). ABCC4 localizes to both plasma membrane and intracellular membranous structures (10). Investigators have also implicated ABCC4 in the pathogenesis of Kawasaki disease, a childhood genetic disorder characterized by vasculitis (11).

1. Nakanishi, T. Cancer Genomics Proteomics 4, 241-54.
2. Kool, M. et al. (1997) Cancer Res 57, 3537-47.
3. Lee, K. et al. (1998) Cancer Res 58, 2741-7.
4. Nies, A.T. et al. (2004) Neuroscience 129, 349-60.
5. Giacomini, K.M. et al. (2010) Nat Rev Drug Discov 9, 215-36.
6. Copsel, S. et al. (2011) J Biol Chem 286, 6979-88.
7. Omura, N. et al. (2010) Mol Cancer Res 8, 821-32.
8. Bronger, H. et al. (2005) Cancer Res 65, 11419-28.
9. Hagmann, W. et al. (2009) Pancreatology 9, 136-44.
10. Rius, M. et al. (2008) J Pharmacol Exp Ther 324, 86-94.
11. Khor, C.C. et al. (2011) J Med Genet 48, 467-72.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150
mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02%
sodium azide. Store at –20°C. Do not aliquot the antibody.
*Species cross-reactivity is determined by western blot.
** Anti-rabbit secondary antibodies must be used to
detect this antibody.