Plexin Isoform Antibody Sampler Kit

• 产品编号：CST-12823S      品牌：Cell Signaling Technology       原厂货号：12823S
• 产品分类：抗体 > 一抗 > 复合抗体试剂盒
• 应用分类：

其它组分：

描述：

Plexin A1 Antibody 兔多抗可以识别内源性的Plexin A1总蛋白。Plexin A2 (D42B5) Rabbit mAb 兔单抗可以识别内源性的Plexin A2总蛋白，可能和不同分子量的未知源性蛋白发生交叉反应。Plexin A3 (D2G12) Rabbit mAb 兔单抗可以检测到内源性的Plexin A3总蛋白，并可能和其他家族成员发生交叉反应。多克隆抗体由合成的人Plexin A1蛋白Gln1875邻近肽段免疫动物而获得，并通过蛋白A和多肽亲和层析纯化。单克隆抗体由合成的人Plexin A2蛋白Gly185邻近肽段，或人Plexin A3蛋白Val440邻近肽段，或人Plexin A4蛋白邻近肽段的GST融合蛋白，分别免疫动物而获得。Plexin Isoform Antibody Sampler Kit小包装抗体试剂盒提供了一种经济的方式检测Plexin A1, A2, A3 和A4蛋白的表达水平。该试剂盒包含了足够的一抗以满足每款抗体用于4张膜的免疫印迹实验。分泌型壁板蛋白类别3（Class 3 secreted semaphorin，Sema3A）是多种轴突上的神经生长导向因子。因此，它可以诱导微丝发生戏剧性的再分布和解聚，导致生长锥崩塌。Plexins是单次跨膜信号转导蛋白，包括Plexin A1，A2，A3和A4。Plexins可以与neuropilin-1、neuropilin-2和细胞黏附蛋白L1形成功能性的semaphorin受体（1,2）。GTP酶Rnd1结合Plexin A1的细胞质结构域，触发细胞骨架崩溃。相反，GTP酶RndD阻止Rnd1-介导的Plexin A1的活性和Sema3A所致交感神经轴突的排斥（3）。Sema6A是Plexin A2的配体。Sema6A 和 Plexin A2敲除小鼠都有颗粒细胞迁移障碍，细胞都位于分子层。此外，Plexin A2一控制了细胞核-中心体偶联以调控细胞迁移(4)。Plexin A3和A4介导了感觉和交感神经元class 3 semaphorins的应激反应。特别的是，Plexin A4通过neuropilin-1负责Sema 3A信号通路，而Plexin A3通过neuropilin-2负责Sema 3A信号(5)。

原厂资料：

Specificity / Sensitivity

Plexin A1 Antibody detects endogenous levels of total Plexin A1 protein. Plexin A2 (D42B5) Rabbit mAb detects endogenous levels of total Plexin A2 protein and may cross-react with unidentified proteins at various molecular weights. Plexin A3 (D2G12) Rabbit mAb detects endogenous levels of total Plexin A3 protein and may cross-react with other family members. Plexin A4 (C5D1) Rabbit mAb detects endogenous levels of total Plexin A4 protein.

Source / Purification

Polyclonal antibody is produced by immunizing animals with a synthetic peptide surrounding Gln1875 of human Plexin A1 protein and purified by protein A and peptide affinity chromatography. Monoclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly185 of human Plexin A2 protein, or Val440 of human Plexin A3 protein, or with a GST-fusion protein corresponding to residues from the human sequence of Plexin A4 protein.

Description

The Plexin Isoform Antibody Sampler Kit provides an economical means of evaluating Plexin A1, A2, A3 and A4 protein expression. The kit contains enough primary antibody to perform four western miniblot experiments per primary antibody.

Background

Class 3 secreted semaphorin (Sema3A) is a chemorepellent that acts upon a wide variety of axons. As such, it induces a dramatic redistribution and depolymerization of actin filaments that results in growth cone collapse. Plexins are single membrane-spanning signaling proteins encompassing Plexin A1, A2, A3, and A4. Plexins form a complex with neuropilin-1 and -2 and the cell adhesion protein L1 to form a functional semaphorin receptor (1,2). The GTPase Rnd1 binds to the cytoplasmic domain of Plexin A1 to trigger cytoskeletal collapse. In contrast, the GTPase RhoD blocks Rnd1-mediated Plexin A1 activation and repulsion of sympathetic axons by Sema3A (3). Sema6A is a ligand for Plexin A2. Both Sema6A and Plexin A2 knock-out mice have a granule cell migration defect, where cells remain in the molecular layer. Furthermore, Plexin A2 also controls nucleus-centrosome coupling that modulates cell migration (4). Both Plexin A3 and A4 mediate the responses to class 3 semaphorins in sensory and sympathetic neurons. In particular, Plexin A4 is responsible for signaling of Sema 3A via neuropilin-1, while Plexin A3 is responsible for signaling of Sema 3F via neuropilin-2 (5).

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150
mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02%
sodium azide. Store at –20°C. Do not aliquot the antibodies.
Recommended Antibody Dilutions:
Western blotting  1:1000