SSU72 (D3I2D) Rabbit mAb

• 产品编号：CST-12816S      品牌：CST       原厂货号：12816S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

SSU72 (D3I2D) Rabbit mAb兔单抗能够检测内源性SSU72总蛋白水平。该单克隆抗体采用合成的与人源SSU72蛋白Pro93残基周围序列相对应的肽段免疫动物而生产的。SSU72是一种蛋白磷酸酶，最初是通过与TFIIB的相互作用鉴定出来的，是转录和RNA加工所需要的因子（1,2）。而SSU72还作为RNA聚合酶II 羧基端结构域中七肽重复序列的Ser5位点的磷酸酶而为大家所熟知，此外还有一些研究暗示SSU72还能够磷酸化七肽重复序列中的Ser7位点。（3-9）。SSU72的活性还可以将转录与前体mRNA 3’ 末端加工结合在一起（10-12）。在将基因启动子和终止子区域联系起来的基因成环过程中需要SSU72的参与，从而产生正确的转录起始并且增强在双向启动子上的转录方向（13-14）。除了在转录中的作用，SSU72还能作为黏连蛋白结合蛋白来调控有丝分裂或减数分裂过程中在姐妹染色臂之间的黏连蛋白（15）。

1. Sun, Z.W. and Hampsey, M. (1996) Mol Cell Biol 16, 1557-66.
2. Pappas, D.L. and Hampsey, M. (2000) Mol Cell Biol 20, 8343-51.
3. Ganem, C. et al. (2003) EMBO J 22, 1588-98.
4. Krishnamurthy, S. et al. (2004) Mol Cell 14, 387-94.
5. Hausmann, S. et al. (2005) J Biol Chem 280, 37681-8.
6. Reyes-Reyes, M. and Hampsey, M. (2007) Mol Cell Biol 27, 926-36.
7. Werner-Allen, J.W. et al. (2011) J Biol Chem 286, 5717-26.
8. Zhang, D.W. et al. (2012) J Biol Chem 287, 8541-51.
9. Bataille, A.R. et al. (2012) Mol Cell 45, 158-70.
10. He, X. et al. (2003) Genes Dev 17, 1030-42.
11. Steinmetz, E.J. and Brow, D.A. (2003) Mol Cell Biol 23, 6339-49.
12. Xiang, K. et al. (2010) Nature 467, 729-33.
13. Wang, Y. et al. (2010) Curr Biol 20, 548-53.
14. Tan-Wong, S.M. et al. (2012) Science 338, 671-5.
15. Kim, H.S. et al. (2010) EMBO J 29, 3544-57.

原厂资料：

Homology

Species predicted to react based on 100% sequence homology: Hamster, Chicken, Bovine, Dog, Pig, Guinea Pig

Specificity / Sensitivity

SSU72 (D3I2D) Rabbit mAb recognizes endogenous levels of total SSU72 protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro93 of human SSU72 protein.

Background

SSU72 is a protein phosphatase originally identified through its interaction with TFIIB as a factor required for transcription and RNA processing (1,2). While SSU72 has been known as a phosphatase of the RNA polymerase II carboxy-terminal domain at Ser5 of the heptapeptide repeat, additional studies suggest a role for SSU72 as a phosphatase for Ser7 of the heptapeptide repeat as well (3-9). The activities of SSU72 are thought to play a critical role in coupling transcription with pre-mRNA 3’ end processing (10-12). SSU72 is required for gene looping that connects the gene promoter with the terminator region, leading to proper initiation of transcription and enforcement of transcriptional directionality on promoters that may other wise act as bidirectional promoters (13-14). In addition to a role in transcription, SSU72 acts as a cohesin binding protein that regulates cohesion between sister chromatid arms during mitosis or meiosis (15).

1. Sun, Z.W. and Hampsey, M. (1996) Mol Cell Biol 16, 1557-66.
2. Pappas, D.L. and Hampsey, M. (2000) Mol Cell Biol 20, 8343-51.
3. Ganem, C. et al. (2003) EMBO J 22, 1588-98.
4. Krishnamurthy, S. et al. (2004) Mol Cell 14, 387-94.
5. Hausmann, S. et al. (2005) J Biol Chem 280, 37681-8.
6. Reyes-Reyes, M. and Hampsey, M. (2007) Mol Cell Biol 27, 926-36.
7. Werner-Allen, J.W. et al. (2011) J Biol Chem 286, 5717-26.
8. Zhang, D.W. et al. (2012) J Biol Chem 287, 8541-51.
9. Bataille, A.R. et al. (2012) Mol Cell 45, 158-70.
10. He, X. et al. (2003) Genes Dev 17, 1030-42.
11. Steinmetz, E.J. and Brow, D.A. (2003) Mol Cell Biol 23, 6339-49.
12. Xiang, K. et al. (2010) Nature 467, 729-33.
13. Wang, Y. et al. (2010) Curr Biol 20, 548-53.
14. Tan-Wong, S.M. et al. (2012) Science 338, 671-5.
15. Kim, H.S. et al. (2010) EMBO J 29, 3544-57.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150
mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02%
sodium azide. Store at –20°C. Do not aliquot the antibody.