CYLD (D6O5O) Rabbit mAb

• 产品编号：CST-12797S      品牌：CST       原厂货号：12797S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

CYLD (D6O5O) Rabbit mAb识别内源性的CYLD总蛋白。该单克隆抗体由重组的人CYLD蛋白免疫动物产生。CYLD是一种由一种肿瘤抑制基因编码的细胞质去泛素化酶，在圆柱瘤患者中该基因发生改变，该疾病是一种由基因变化引起的皮肤附件的良性肿瘤(1,2)。功能性的CYLD去泛素化酶，通过去除几种NF-κB通路蛋白的泛素链，下调NF-κB信号转导，进而调节炎症和细胞增殖(3,4)。CYLD是Wnt/β-catenin信号转导中邻近事件的一种负向调节剂，并是自然杀伤T细胞发育中的一种重要的调节剂(5,6)。转录因子Snail能够抑制CYLD的表达，从而导致黑色素瘤细胞增殖(7)。

1. Biggs, P.J. et al. (1995) Nat Genet 11, 441-3.
2. Sun, S.C. (2010) Cell Death Differ 17, 25-34.
3. Regamey, A. et al. (2003) J Exp Med 198, 1959-64.
4. Glittenberg, M. and Ligoxygakis, P. (2007) Fly (Austin) 1, 330-2.
5. Tauriello, D.V. et al. (2010) Mol Cell 37, 607-19.
6. Lee, A.J. et al. (2010) EMBO J 29, 1600-12.
7. Massoumi, R. et al. (2009) J Exp Med 206, 221-32.

原厂资料：

Specificity / Sensitivity

CYLD (D6O5O) Rabbit mAb recognizes endogenous levels of total CYLD protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with recombinant human CYLD protein.

Background

CYLD is a cytoplasmic deubiquitinating enzyme encoded by a tumor suppressor gene altered in individuals diagnosed with cylindromatosis, a genetic condition characterized by benign tumors of skin appendages (1,2). Functional CYLD deubiquitinase regulates inflammation and cell proliferation by down regulating NF-κB signaling through removal of ubiquitin chains from several NF-κB pathway proteins (3,4). CYLD is a negative regulator of proximal events in Wnt/β-catenin signaling and is a critical regulator of natural killer T cell development (5,6). The transcription factor Snail can inhibit CYLD expression, resulting in melanoma cell proliferation (7).

1. Biggs, P.J. et al. (1995) Nat Genet 11, 441-3.
2. Sun, S.C. (2010) Cell Death Differ 17, 25-34.
3. Regamey, A. et al. (2003) J Exp Med 198, 1959-64.
4. Glittenberg, M. and Ligoxygakis, P. (2007) Fly (Austin) 1, 330-2.
5. Tauriello, D.V. et al. (2010) Mol Cell 37, 607-19.
6. Lee, A.J. et al. (2010) EMBO J 29, 1600-12.
7. Massoumi, R. et al. (2009) J Exp Med 206, 221-32.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150
mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02%
sodium azide. Store at –20°C. Do not aliquot the antibody