Glutamate Dehydrogenase 1/2 (D9F7P) Rabbit mAb

• 产品编号：CST-12793S      品牌：CST       原厂货号：12793S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

Glutamate Dehydrogenase 1/2 (D9F7P) Rabbit mAb 兔单抗可以识别内源性的总谷氨酸脱氢酶1和 2蛋白。仅在人体中会发生IHC-P和IF-IC的交叉反应。该单克隆抗体由合成的人谷氨酸脱氢酶1蛋白Pro190附近肽段免疫动物而获得。谷氨酸脱氢酶是一种线粒体酶通过辅助因子烟酰胺腺嘌呤二核苷酸磷酸催化谷氨酸氧化脱氨基形成α-酮戊二酸(1)。谷氨酸脱氢酶在多种组织中高表达，例如肝脏，大脑，肾脏，心脏，胰脏，卵巢和睾丸。哺乳动物组织中发现由两个不同的基因会形成两种亚型。GLUD1基因是广泛表达的（2），而GLUD2基因特异性表达在睾丸组织和星形胶质细胞中(3,4)。谷氨酸脱氢酶使谷氨酸盐进入三羧酸循环，因此在能量稳态调节过程中发挥了关键的调控作用。研究表明胰岛β细胞中谷氨酸脱氢酶活性变化会导致高胰岛素血症综合征(5)。

1. Blumenthal, K.M. et al. (1975) J Biol Chem 250, 3644-54.
2. Michaelidis, T.M. et al. (1993) Genomics 16, 150-60.
3. Shashidharan, P. et al. (1997) J Neurochem 68, 1804-11.
4. Zaganas, I. et al. (2012) Neurochem Int 61, 455-62.
5. Karaca, M. et al. (2011) Neurochem Int 59, 510-7.

原厂资料：

Specificity / Sensitivity

Glutamate Dehydrogenase 1/2 (D9F7P) Rabbit mAb recognizes endogenous levels of total glutamate dehydrogenase 1 and 2 proteins. Species cross-reactivity for IHC-P and IF-IC is in human only.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro190 of human glutamate dehydrogenase 1 protein.

Background

Glutamate dehydrogenase is a mitochondrial enzyme that catalyzes the oxidative deamination of glutamate to α-ketoglutarate through association with the cofactor nicotinamide adenine dinucleotide phosphate (1). Glutamate dehydrogenase is highly expressed in various tissues such as the liver, brain, kidney, heart, pancreas, ovaries, and testis. Two isoforms produced by two distinct genes are found in mammalian tissues. The GLUD1 gene is ubiquitously expressed (2), while the GLUD2 gene is specifically expressed in testicular tissues and astrocytes (3,4). Glutamate dehydrogenase links glutamate to the Krebs cycle, thereby playing a critical role in the regulation of energy homeostasis. Research studies have shown that changes in glutamate dehydrogenase activity in pancreatic β-cells can cause a hyperinsulinism syndrome (5).

1. Blumenthal, K.M. et al. (1975) J Biol Chem 250, 3644-54.
2. Michaelidis, T.M. et al. (1993) Genomics 16, 150-60.
3. Shashidharan, P. et al. (1997) J Neurochem 68, 1804-11.
4. Zaganas, I. et al. (2012) Neurochem Int 61, 455-62.
5. Karaca, M. et al. (2011) Neurochem Int 59, 510-7.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150
mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02%
sodium azide. Store at –20°C. Do not aliquot the antibody.