SHMT2 Antibody

• 产品编号：CST-12762S      品牌：CST       原厂货号：12762S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

多抗由合成肽段免疫动物产生，该肽段与人SHMT2蛋白Phe441附近氨基酸一致。抗体由蛋白A和肽段亲和层析技术纯化得到。Serine hydroxymethyltransferases 1和2 (SHMT1, SHMT2)分别是细胞质和线粒体serine hydroxylmethyltransferases(1,2)。它们催化丝氨酸转化成甘氨酸和β-碳从丝氨酸转移到四氢叶酸（THF）中，以形成5，10 - 亚甲基 - 十二烷基硫酸钠(1, 2)。研究表明SHMT1半合子与某种遗传背景小鼠高风险的肠癌罹患率相关(3)。抑制SHMT2会阻断细胞扩增(4)。

1. MacFarlane, A.J. et al. (2008) J Biol Chem 283, 25846-53.
2. Hebbring, S.J. et al. (2012) J Neurochem 120, 881-90.
3. Macfarlane, A.J. et al. (2011) Cancer Res 71, 2098-107.
4. di Salvo, M.L. et al. (2013) Med Hypotheses 80, 633-6.

原厂资料：

Specificity / Sensitivity

SHMT2 Antibody recognizes endogenous levels of total SHMT2 protein.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Phe441 of human SHMT2 protein. Antibodies are purified by protein A and peptide affinity chromatography.

Background

Serine hydroxymethyltransferases 1 and 2 (SHMT1, SHMT2) are cytoplasmic and mitochondrial serine hydroxylmethyltransferases, respectively (1,2). They catalyze the conversion of serine to glycine with the transfer of β-carbon from serine to tetrahydrofolate (THF) to form 5, 10-methylene-THF (1, 2). Research studies indicate that SHMT1 hemizygosity is associated with higher risk of intestinal cancer in mice of a certain genetic background (3). Suppression of SHMT2 was shown to block cell proliferation (4).

1. MacFarlane, A.J. et al. (2008) J Biol Chem 283, 25846-53.
2. Hebbring, S.J. et al. (2012) J Neurochem 120, 881-90.
3. Macfarlane, A.J. et al. (2011) Cancer Res 71, 2098-107.
4. di Salvo, M.L. et al. (2013) Med Hypotheses 80, 633-6.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM
NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C.
Do not aliquot the antibody.