NRF2 (D1Z9C)?XP? Rabbit mAb

• 产品编号：CST-12721P      品牌：Cell Signaling Technology       原厂货号：12721P
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

Nuclear factor-like 2 (NRF2)转录激活因子能够结合靶基因启动子区域的antioxidant response elements (ARE)，从而调节氧化应激反应基因的表达。在生理条件下，NRF2抑制因子INrf2 (又称KEAP1)结合NRF2并使NRF2保持在细胞质中，从而使NRF2通过泛素化通路介导降解(1)。少量组成型NRF2通过抗氧化反应基因的基本表达的调控来维持细胞内稳定。在氧化应激或亲电子反应后，KEAP1蛋白释放NRF2蛋白，从而允许该转录激活因子进入细胞核，并且结合到靶基因的ARE元件(2)。NRF2蛋白和其它转录因子协同介导氧化应激反应(3)。NRF2蛋白的可变表达与chronic obstructive pulmonary disease (COPD)有关(4)。在肺癌细胞系中NRF2蛋白的活性直接与细胞增殖率有关，同时通过siRNA沉默抑制NRF2蛋白表达能够提高抗癌药物介导的凋亡(5)。

1. Cullinan, S.B. et al. (2004) Mol Cell Biol 24, 8477-86.
2. Nguyen, T. et al. (2005) J Biol Chem 280, 32485-92.
3. Jaiswal, A.K. (2004) Free Radic Biol Med 36, 1199-207.
4. Suzuki, M. et al. (2008) Am J Respir Cell Mol Biol 39, 673-82.
5. Homma, S. et al. (2009) Clin Cancer Res 15, 3423-32.

原厂资料：

Specificity / Sensitivity

NRF2 (D1Z9C) XP^® Rabbit mAb recognizes endogenous levels of total NRF2 protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ala275 of human NRF2 protein.

Background

The nuclear factor-like 2 (NRF2) transcriptional activator binds antioxidant response elements (ARE) of target gene promoter regions to regulate expression of oxidative stress response genes. Under basal conditions, the NRF2 inhibitor INrf2 (also called KEAP1) binds and retains NRF2 in the cytoplasm where it can be targeted for ubiquitin-mediated degradation (1). Small amounts of constitutive nuclear NRF2 maintain cellular homeostasis through regulation of basal expression of antioxidant response genes. Following oxidative or electrophilic stress, KEAP1 releases NRF2, thereby allowing the activator to translocate to the nucleus and bind to ARE-containing genes (2). The coordinated action of NRF2 and other transcription factors mediates the response to oxidative stress (3). Altered expression of NRF2 is associated with chronic obstructive pulmonary disease (COPD) (4). NRF2 activity in lung cancer cell lines directly correlates with cell proliferation rates, and inhibition of NRF2 expression by siRNA enhances anti-cancer drug-induced apoptosis (5).

1. Cullinan, S.B. et al. (2004) Mol Cell Biol 24, 8477-86.
2. Nguyen, T. et al. (2005) J Biol Chem 280, 32485-92.
3. Jaiswal, A.K. (2004) Free Radic Biol Med 36, 1199-207.
4. Suzuki, M. et al. (2008) Am J Respir Cell Mol Biol 39, 673-82.
5. Homma, S. et al. (2009) Clin Cancer Res 15, 3423-32.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150
mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02%
sodium azide. Store at –20°C. Do not aliquot the antibody.